Protein

Profilin (Synonyms: Profilin 1)

Origin

human recombinant - untagged.

Molecular mass

15kDa

Protein description

Profilin (15kDa) regulates the G-actin pool by sequestration of actin monomers. Thus profilin controls the growth of actin filaments, and is thus an essential regulator of cellular locomotion and cell shape changes.

Profilin is negatively regulated by phospholipids (PIP2, InsP3), reversed by phospholipase C.

Actin interaction

Profilin binds to monomeric actin by occupying an actin-actin interaction site. As a result, profilin sequesters actin from the G-actin pool. Profilin catalyzes the exchange of actin-bound ADP to ATP. This converts less polymerizable ADP-actin into fast polymerizable ATP-actin. Since profilin has a significantly higher affinity for ATP- than for ADP-actin.

Another F-actin growth control by profiling is that profilin-actin complexes are introduced into growing actin polymers by e.g. formin, WASP and VASP. 

Form

Lyophilized, ready-to-use.

Quantity  per unit

2x50 µg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 50µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity 

>95% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Profilin protein was expressed in E. coli and further purified by Affinity Chromatography (Polyproline column). The pure protein was yielded by gel filtration. 

Protein concentration

Determined by OD280 (0.1%=1.226).

Storage instructions

Profilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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