Profilin (human recombinant) - 2x50µg
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Actin Binding Protein
Profilin (Profilin 1)
(Actin Binding Protein)
Cat. #: 8418-01
Description
| Protein | Profilin (Synonyms: Profilin 1) |
| Origin | Human recombinant, untagged |
| Molecular mass | 15 kDa |
| Protein description | Profilin (15 kDa) regulates the G-actin pool by sequestration of actin monomers and thereby controls actin filament growth, a key determinant of cell motility and shape changes. Profilin activity is negatively regulated by phospholipids (PIP2, InsP3) and can be reversed by phospholipase C. |
| Actin interaction | Profilin binds monomeric actin by occupying an actin–actin interaction site, thereby sequestering actin from the monomer pool. Profilin catalyzes nucleotide exchange on actin (ADP to ATP), converting less polymerizable ADP-actin to more readily polymerizable ATP-actin, consistent with a higher affinity for ATP-actin than for ADP-actin. Profilin–actin complexes can be delivered to growing filament ends via elongation factors such as formins, WASP and VASP. |
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 2 x 50 µg |
| Buffer | 150 mM NaCl, 20 mM HEPES pH 7.4, 1 mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution. |
| Purity | >95% by scanning densitometry from Coomassie G-250 stained SDS gels. |
| Purification notes | Expressed in E. coli and purified by affinity chromatography (polyproline column), followed by gel filtration. |
| Protein concentration | Determined by OD280 (0.1% = 1.226). |
| Storage instructions | Store at -70°C upon arrival. The product is stable in performance for at least 6 months from the date of purchase when stored at -70°C. After reconstitution keep on ice during use and store at -20°C as a glycerol stock or in CryoProtect (Cat. #: 6011-01). Avoid repeated freeze / thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P. Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P. Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Structure of actin binding proteins: insights about function at atomic resolution.
Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE. Annu Rev Cell Biol. 1994;10:207-49. Review.
Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE. Annu Rev Cell Biol. 1994;10:207-49. Review.
Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells.
Carlsson L, Nyström LE, Sundkvist I, Markey F, Lindberg U. J Mol Biol. 1977 Sep 25;115(3):465-83.
Carlsson L, Nyström LE, Sundkvist I, Markey F, Lindberg U. J Mol Biol. 1977 Sep 25;115(3):465-83.
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
Protein Sequences and Structures on RCSB
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Structure of actin binding proteins: insights about function at atomic resolution.
Pollard TD, Almo S, Quirk S, Vinson V, Lattman EE.
Annu Rev Cell Biol. 1994;10:207-49. Review.
Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells.
Carlsson L, Nyström LE, Sundkvist I, Markey F, Lindberg U.
J Mol Biol. 1977 Sep 25;115(3):465-83.
