ATTO647-Actin
α-actin from skeletal muscle, rabbit
Cat. #: 8158-01
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Applications
Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.
Description
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Protein |
ATTO647-Actin |
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Origin |
Skeletal muscle alpha actin, rabbit |
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Molecular mass |
42kDa |
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Protein description |
Actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. ATTO647-Actin is chemically modified G-actin with an ATTO647 fluorescent group covalently bound to lysine residues.
ATTO647-Actin (λex 647nm, λem669nm) possesses the polymerization properties of native G-actin. |
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Conjugation ratio |
This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. The precise DOL is stated in the DataSheet supplied with the product. |
Properties
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Form |
Lyophilized, ready-to-use. |
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Quantity per unit |
2x100 µg |
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Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution. |
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Purity |
>99% by scanning densitometry. |
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Purification notes |
Purified by GPC from rabbit skeletal muscle. |
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Protein concentration |
Determined by the Biuret method. |
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Storage instructions |
ATTO647-Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized product is kept on ice, protect from light and it should be used within 1 week.Avoid refreezing of actin. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
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Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI
References
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.
Product DataSheetMaterial and Safety Data SheetTable of Dyes and Light Sources
Protein Sequence on NCBI
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-53. doi: 10.1038/ncb3402. Epub 2016 Aug 22.
