Antibodies
ATTO dye–conjugated secondary antibodies
For fluorescence microscopy, the dominant failure modes are photobleaching, background, and spectral cross-talk. Hypermol® ATTO-conjugated secondary antibodies are built for bright, photostable signal and clean multiplex performance across standard and high-resolution imaging. They are suited for immunofluorescence applications where stable intensity and reproducibility matter.
Typical use cases include cell and tissue imaging, high-content screening, and multicolor immunostaining. For specific assay performance, primary antibody affinity, fixation/permeabilization, and microscope configuration are key determinants of signal quality.
| Green | ~488 nm excitation / ~500–550 nm emission (FITC/GFP-compatible channel). |
| Red | ~561 nm excitation / ~570–620 nm emission (TRITC/Texas Red-like channel). |
| Far-red | ~633–640 nm excitation / ~650–750 nm emission (Cy5/Alexa 647-like channel). |
Primary antibodies
We provide a selection of validated primary antibodies against cytoskeletal proteins for workflows such as immunoblotting (Western blot), immunoprecipitation, and immunocytochemistry / immunofluorescence. Performance in microscopy depends on antigen accessibility (fixation), epitope preservation, and background suppression.
Accessories
Complementary reagents for reliable staining and imaging workflows, including actin staining kits, embedding media, and blocking solutions.
Antibodies – fluorescent secondary antibodies and imaging accessories from Hypermol®
Secondary antibodies are widely used reporter reagents in immunoassays. In immunofluorescence, a fluorophore-conjugated secondary antibody binds to the primary antibody and enables detection on standard fluorescence microscopes using appropriate filter sets. In immunoblotting, secondaries are commonly used to visualize antibody-bound targets via labeled detection formats.
Hypermol® offers ATTO dye–conjugated secondary antibodies for fluorescence microscopy workflows where brightness, photostability, and clean channel separation are critical. For multiplex experiments, select conjugates by excitation/emission compatibility and your instrument’s filters, and verify performance with your fixation, permeabilization, and blocking conditions.
Fluorescence channels (typical excitation / emission windows)
| Green | ~488 nm excitation / ~500–550 nm emission (FITC/GFP-like channel). |
| Red | ~561 nm excitation / ~570–620 nm emission (TRITC/Texas Red-like channel). |
| Far-red | ~633–640 nm excitation / ~650–750 nm emission (Cy5/Alexa 647-like channel). |
Secondary antibodies for research – practical selection guidance
For robust immunofluorescence results, match your secondary antibody to the host species and isotype of the primary antibody, and choose a fluorophore that fits your imaging panel (single-color or multiplex). Signal quality is strongly influenced by sample preparation (fixation/permeabilization), blocking strategy, primary antibody concentration, and imaging settings.
For multicolor staining, prioritize spectral separation (filters, dichroics, detectors) and, when necessary, use far-red channels to reduce autofluorescence in challenging samples. If your experiment uses multiple primaries, consider species/isotype planning to avoid cross-reactivity and simplify secondary selection.
Actin staining and accessories for fluorescence microscopy
Browse microscopy-ready accessories in the Hypermol® e-shop, including actin staining kits for cellular F-actin visualization, blocking solutions, and embedding media (with or without nuclear counterstains depending on the workflow). 
Reduce photobleaching in fluorescence microscopy
If you observe fading fluorescence, use an antifade strategy appropriate for your dyes and imaging mode. Photobleaching is driven by illumination intensity, exposure time, oxygen chemistry, and the local microenvironment of the fluorophore. In practice, optimizing illumination and using compatible antifade reagents are often the most effective levers for improving image stability.
Quality and support
Hypermol® supplies antibodies and microscopy reagents for reproducible research workflows. If you share your primary antibody species/isotype, sample type, and microscope channels, we can help you choose a compatible secondary antibody and staining setup.
Contact: cusserv@hypermol.com