HY-ImmunoBlock (IF-Blocking Reagent)
Protein-free polymer-based blocking solution for immunofluorescence
Cat. #: 6078-01
Applications
Highly suitable for immunofluorescence (IF), super-resolution microscopy (STORM, PALM), and multiplex staining procedures. Compatible with sensitive detection systems. Reduces background fluorescence and non-specific binding.
With 250 ml of working solution, you can typically:
- Block over 5,000 microscopy spots on glass slides (50 µl/spot)
- Treat more than 800 coverslips (18 mm Ø, 300 µl)
- Block up to 25 full 96-well plates (100 µl/well)
This solution is optimized for high-sensitivity immunofluorescence. It effectively reduces non-specific binding and background fluorescence — even in demanding super-resolution applications.
Description
| Product name |
HY-ImmunoBlock (IF-Blocking Reagent) |
| Application |
Immunofluorescence, super-resolution, multiplex IF, all types of IF plate assays |
| Description |
HY-ImmunoBlock is a 5× polymer-based blocking buffer optimized for high-sensitivity immunofluorescence techniques. It contains synthetic, serum-free polymers such as PVA and PVP for effective background reduction.
The buffer minimizes non-specific antibody interactions and is suitable for use with a wide variety of primary and secondary antibody systems. It is compatible with fluorophores and does not autofluoresce.
|
| Specification |
Use at 1:5 dilution in PBS. Ready-to-use after dilution. Do not freeze. |
| Composition |
Protein-free polymer based proprietary formula with 0.05 % NaN₃ in PBS |
Properties
| Form |
Liquid |
| Volume |
250 ml (50ml, 5× concentrate) |
| Storage |
2–8 °C, protected from light. Do not freeze. |
| Shelf life |
Minimum 1 year. |
| Shipping |
Ambient temperature |
| Remarks |
For research use only. Not for human or veterinary diagnostic or therapeutic use. |
Buffers and reagents from Hypermol® are produced using the purest components and ultrapure Milli-Q™ water.
