FluMaXx (Oxygen scavenger for single molecule imaging)
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FluMaXx
Oxygen scavenging system for single-molecule fluorescence imaging; 2.5 ml, sufficient for approximately 100–200 assays.
Applications
| Applications | Oxygen scavenging and antifade system for fluorescence microscopy, single-molecule fluorescence imaging and flow-chamber protein interaction assays. |
| Recommended use | FluMaXx is recommended for assays requiring strong photoprotection and stable fluorescence signals. It is suitable for workflows using fluorescent dyes, fluorescently labeled proteins and cytoskeletal polymers that are sensitive to photobleaching or photon-induced chemical damage. |
| Microscopy methods | Suitable for fluorescence microscopy and spectroscopy workflows including TIRFM, epifluorescence microscopy, GSD, STED and dSTORM, provided that the complete assay conditions are compatible with the sample and detection method. |
Description
| Product name | FluMaXx |
| Product description | FluMaXx is an oxygen scavenging system designed to improve fluorescence stability and reduce photo-induced damage during fluorescence microscopy and single-molecule imaging. The system is based on an optimized glucose oxidase/catalase formulation and is supplied as a lyophilized multi-component system for controlled reconstitution. By lowering dissolved oxygen in the assay environment, oxygen scavenging systems can reduce photobleaching and photochemical damage of fluorescent dyes and fluorescently labeled biomolecules. FluMaXx is particularly useful for high-resolution monitoring of assays using cytoskeletal polymers, where fluorescent filaments may be susceptible to photon-induced chemical damage and fragmentation during prolonged observation. The product contains the required components for fluorescence-preserving imaging buffer preparation, including Sol. A, Sol. B and methylcellulose stock solution. Sol. A contains the glucose oxidase/catalase enzyme system and buffer components; Sol. B contains ATP and DTT and is added freshly before use. |
| Overview | High-performance oxygen scavenger for prolonged fluorescence, reduced protein damage and reduced fragmentation of fluorescent cytoskeletal polymers; supplied as lyophilized Sol. A and Sol. B with methylcellulose stock solution; final product volume 2.5 ml. |
| Related workflow | FluMaXx is intended as an imaging buffer supplement for fluorescence-based assays. It can be used in combination with actin, cytoskeletal polymers, actin-binding proteins or fluorescence-labeled assay components when oxygen scavenging and antifade protection are required. |
Properties
| Form | Lyophilized three-component system, ready-to-use after reconstitution. The product includes methylcellulose stock solution supplied as 0.5% solution. |
| Components | 5 × 500 µl Sol. A, lyophilized. Contains glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2, glucose and cryoprotectants in a proprietary formulation. 1 × 500 µl Sol. B, lyophilized. Contains ATP and DTT. 1 × 1.5 ml methylcellulose stock solution, 0.5% in Milli-Q™ water. |
| Final product volume | 2.5 ml, sufficient for approximately 100–200 assays. |
| Reconstitution and use | For flow-chamber assays, add 250 µl ultrapure water to one tube labelled Sol. A and mix with 200 µl methylcellulose stock solution. After addition of methylcellulose to Sol. A, vortex the mixture and centrifuge for 1 min at 5000 rpm. Add Sol. B freshly before combining the imaging buffer with the sample solution to be observed. A typical mixing example is 45 µl Sol. A/methylcellulose mixture plus 5 µl Sol. B. For use as an antifade buffer system without methylcellulose addition, add 450 µl ultrapure water to one tube labelled Sol. A. Mix Sol. B shortly before use, for example 45 µl Sol. A plus 5 µl Sol. B. |
| Handling note | Short degassing before use is recommended to remove trapped air from viscous FluMaXx mixtures. |
| Purity | All components are prepared in Milli-Q™ water and 0.2 µm filtered. |
| Storage instructions | FluMaXx stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. After reconstitution, aliquots of FluMaXx, Sol. A/methylcellulose mixture and Sol. B can be prepared for storage. Store on ice after reconstitution and use final mixtures within 1 day. Prepare aliquots immediately after reconstitution for deep-freezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Pergola C, Schubert K, Pace S, Ziereisen J, Nikels F, Scherer O, Hüttel S, Zahler S, Vollmar AM, Weinigel C, Rummler S, Müller R, Raasch M, Mosig A, Koeberle A, Werz O. Modulation of actin dynamics as potential macrophage subtype-targeting anti-tumour strategy. Sci Rep. 2017 Jan 30;7:41434. doi: 10.1038/srep41434.
