FluMaXx (Oxygen scavenger for single molecule imaging)

Product name

FluMaXx

Product description

FluMaXx is an fluorescence oxygen scavenging system to improve the stability of fluorescent dyes. The efficiency of FluMaXx is illustrated by its ability to prevent e.g. filamentous actin from fragmentation, which are extremely prone to photon induced chemical damage. 

FluMaXx contains all vital components for high resolution monitoring of assays using cytoskeletal polymers. This optimized catalase/glucose oxidase enzyme system, protects the natural fluorescence intensity of the dye and thus maXximizes the fluorescence intensity.

A novel optimization of FluMaXx is based on the stabilization of the catalyse enzyme. Catalase normally cannot be frozen in solution without a major loss of activity and furthermore is inactivated by higher concentrations of reducing agents. 

Our proprietary formula of the FluMaXx permits refreezing after reconstitution without loss of activity. The buffer contains 5mM DTT, showing a beneficial effect on performance without measurable damage of the enzyme system.      

To guarantee the high quality of FluMaXx, all critical components are supplied as a lyophilized powder.  

Overview

High performance oxygen scavenger to :

• efficiently prolong fluorescence;
• prevent protein damage;
• prevent fragmentation of polymers;

Form

Lyophilized, three-component buffer system, ready-to-use. (Contains methylcellulose as 0.5% solution)

Components






Final product volume

5x 500µl Sol. A (lyophilized)   
Contains: Glucose oxidase, catalase, imidazole pH 7.4, KCl, EGTA, MgCl2, glucose, cryoprotectants (proprietary formula);
1x 500µl Sol. B (lyophilized)
Contains: ATP, DTT 
1x 1.5ml Methylcellulose stock solution (0.5% in Milli-Q™ water)

2.5ml (100-200 assays)

Working instructions

For flow chamber assays: add 250µl of ultrapure water to the tube labelled Sol. A and mix with 200µl of methylcellulose (MC). After addition of the MC-solution to Sol. A, the mixture is vortexed and centrifuged for 1min, 5000rpm.

Sol. B (ATP/DTT-Mix) is added freshly before mixing with the sample solution to be observed (e.g. 45µl Sol. A/MC + 5µl Sol. B).  

It is recommended to shortly degas FluMaXx before use to remove trapped air from the viscous solution.

As antifading buffer system:  add 450µl of ultrapure water to the tube labelled Sol. A . Mix Sol. B shortly before use  (e.g. 45µl Sol. A/MC + 5µl Sol. B).

Purity

All components were made up in Milli-Q™ water and  0.2µm filtered.

Storage instructions

FluMaXx is stored at -70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. After reconstitution aliquots of FluMaXx, Sol. A/MC and Sol. B solutions can be prepared for storage. 

Store on ice after reconstitution and use final mixtures within 1 day. Prepare aliquots immediately after reconstitution for deep freezing. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C. 

Remarks

For Use in Research only. Not for Use in  Human or Veterinary Diagnostical or Therapeutical Applications.

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