Fluorescent Antibody Kit

Anti-Mouse IgG (H+L) ATTO490LS
Antibody produced in goat
Cat. # 2109-1MG
............................................................................................................................................................................................................................................................................................................................................................................................................
Description
|
|
Product name
|
Anti-Mouse IgG ATTO490LS
|
Target species
|
Mouse
|
Description
|
ATTO-labeled secondary antibodies belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG ATTO490LS has a very large stokes shift (165nm). It is thus is specifically suited for suited for all multiplexing approaches, especially in combination with ATTO488 and ATTO514 conjugated antibodies.
The antibody is suited for all applications, including live cell techniques, since it contains no preservatives.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope mapped actin antibody of high performance also on western blots.
|
Specification
|
Goat anti-mouse IgG ATTO490 LS (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
For further information please see DataSheet.
|
Optical Properties
|
λex 496nm, λem 661nm
|
Properties
|
|
Form
|
Lyophilized
|
Nominal concentration
|
2 mg/ml
|
Content
Buffer
|
1 mg Anti-Mouse IgG (H+L) ATTO490LS
20µg (1mg/ml) Monoclonal Anti-Actin - for reference
1.ml Glycerol buffer (to reconstitute the lyophilized antibody)
Certificate of Analysis
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.
|
Clonality
|
Polyclonal
|
Isotype
|
IgG
|
Purification notes
|
Column affinity purified.
|
Storage instructions
|
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
|
Shipping conditions
|
Shipped at ambient temperature.
|
Remarks
|
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
|
CAS no.
|
|
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, Walker LA, Zhao Y, Edwards EM, Michki NS, Cheng HPJ, Ghazzi M, Chen TY, Chen M, Roossien DH, Cai D.
Front Neural Circuits. 2021 Oct 20;15:732183.
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.