Goat Anti-Mouse IgG (H+L) ATTO514
Antibody produced in goat, affinity purified
Cat. #: 2103-1MG
Applications
Suited for multiplexing approaches and live cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Mouse IgG (H+L) ATTO514 | GXM ATTO514 |
| Target species | Mouse |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, distinguished by their exceptional fluorescence intensity, superior photostability, and reliability in diverse applications. Goat Anti-Mouse IgG ATTO514 exhibits a distinct excitation/emission spectrum (λex: 514 nm, λem: 555 nm), providing precise signal detection with minimal spectral overlap, making it particularly effective for multiplex imaging techniques. The antibody’s fluorescence properties, including a high quantum yield and photostability, enable reliable detection of low-abundance targets in both fixed and live samples. The superior brightness and low autofluorescence background ensure clear and reproducible results, even in challenging biological environments. This antibody is preservative-free, allowing its use in sensitive applications like live-cell imaging and fluorescence resonance energy transfer (FRET). Its strong fluorescence signal, minimal non-specific binding, and high performance under various experimental conditions make it a robust tool for advanced research needs. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 514 nm, λem: 555 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) The antibody is shipped in unit sizes of 1.0 mg as a lyophilized powder, shipped at room temperature. To reconstitute the antibody before use, a glycerol buffer is included for convenience. This allows to re-freeze the antibody after use at -20°C, to keep the quality and/or to prepare aliquots, which can be stored at -20°C. |
| Content | 1.0 mg Goat Anti-Mouse IgG (H+L) ATTO514 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgG. Following conjugation to ATTO514 the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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