Anti-Mouse IgG f(ab) ATTO647N (H+L)

Product name

Anti-Mouse IgG f(ab) ATTO647N 

Target species

Mouse

Description

ATTO-labeled f(ab) fragments belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgG f(ab) ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.

Conjugates of f(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access;
- prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in Fab fragments;

Specification

Goat anti-mouse IgG f(ab) ATTO647N (H+L), Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence. 
 

Optical properties

λex 645nm, λem 669nm

Form

Lyophilized

Nominal concentration

2 mg/ml

Content

0.25mg Anti-Mouse IgG f(ab) ATTO647N
1.0 ml Glycerol buffer

Buffer:
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified, IEX chromatography after papain cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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