Goat Anti-Mouse IgG F(ab) ATTO594 (H+L)
F(ab) fragment produced from goat antibody, affinity purified
Cat. #: 2116-250UG
Applications
Suitable for immunofluorescence microscopy and related fluorescence-based assays. The monovalent F(ab) format is smaller than intact IgG and lacks the Fc region, which can improve access to sterically restricted targets and reduce Fc receptor-mediated interactions.
Description
| Product name | Goat Anti-Mouse IgG F(ab) ATTO594 (H+L) | GXM F(ab) ATTO594 |
| Target species | Mouse |
| Description |
Goat Anti-Mouse IgG F(ab) ATTO594 (H+L) is a fluorescent, monovalent secondary-antibody fragment for detecting mouse primary antibodies. The F(ab) fragment is generated from affinity-purified goat anti-mouse IgG by papain cleavage and purified by ion-exchange chromatography. Because the fragment lacks the Fc region, it can reduce Fc receptor-mediated interactions. Its smaller molecular size compared with intact IgG can also be advantageous for sterically restricted epitopes. As a monovalent fragment, F(ab) does not crosslink targets in the same manner as intact IgG or divalent F(ab′)2 fragments. The fragment is conjugated to ATTO594, with an absorption maximum at 603 nm and a fluorescence maximum at 626 nm. Following conjugation, gel filtration removes unbound dye and low-molecular-weight reaction components. |
| Specification | Immunofluorescence: 1:300–1:500, Degree of Labeling (DOL): 1–3, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 603 nm, λfl: 626 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml after reconstitution with 0.125 ml glycerol buffer. |
| Content | 0.25 mg Goat Anti-Mouse IgG F(ab) ATTO594 (H+L) 1.0 ml glycerol buffer supplied Add 0.125 ml buffer to reconstitute the lyophilized fragment to 2 mg/ml. Final buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG-derived F(ab) fragment |
| Purification notes | Parent antibody affinity purified; F(ab) generated by papain cleavage and purified by ion-exchange chromatography (IEX). Following conjugation to ATTO594, the fragment is further purified by gel filtration. |
| Storage instructions | After reconstitution, store at 2–8 °C for up to three months. For extended storage, aliquot and store at −20 °C; avoid repeated freeze/thaw cycles. Protect the fluorescent conjugate from light. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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