Visualize intracellular actin dynamics

A ready-to-start microinjection system for introducing functional fluorescent actin into cultured cells.

The Hypermol^® Actin-Toolkit Microinjection is designed for direct introduction of fluorescent α-actin into normal or transfected cultured cells. Following microinjection, monomeric actin responds to the intracellular ionic environment, polymerizes and becomes incorporated into the cellular actin cytoskeleton, enabling fluorescence-based observation of actin organization and redistribution.

The system is particularly suited to co-localization experiments with transfected actin-binding proteins, analysis of factors affecting cytoskeletal organization and experiments involving pre-formed ligand–actin complexes. Defined ATP-containing actin buffer, filtration units and a fluorescent BSA reference support standardized preparation and control of microinjection experiments.