Actin-Toolkit Microinjection
Rhodamine Cardiac Muscle Alpha Actin, Bovine
Cat. #: 8600-01
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Applications
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Visualization of transfected actin binding proteins affecting the actin cytoskeleton;
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Study of the effects by injection of pre-formed ligand-actin complexes;
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Analysis of components or culturing conditions on the actin cytoskeleton;
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Suitable for use with cell or tissue extracts in biochemical assays;
Description
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Protein |
Rhodamine-Actin |
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Origin |
Bovine cardiac muscle |
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Molecular mass |
42kDa |
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Toolkit description |
The Actin-Toolkit Microinjection all-in-one kit contains rhodamine conjugated α-actin to be microinjected into either normal or transfected cells. For this purpose purified actin was covalently labeled at Cys 374 with tetramethylrhodamine, leaving the polymerization properties of actin essentially unimpaired.
The Actin-Toolkit Microinjection contains 5x100µg Rhodamine-Actin (Rh-Actin), rhodamine conjugated BSA as a reference, ATP-buffers suited for microinjection of actin and mini filter units for the convenient preparation of injectable actin. A handbook is provided with the kit, giving a detailed description of the use of actin for the microinjection technique for a safe and error-free handling of Rhodamine-Actin. |
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Purity |
>99% by scanning densitometry. |
Properties
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Form |
Lyophilized, ready-to-use. |
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Kit content |
5x100µg Rh-Actin (lyophilized powder); |
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Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution. |
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DOL |
>0.5 |
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Purification notes |
Purified from bovine cardiac muscle, GPC. |
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DOL |
Determined by the Biuret method. |
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Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
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Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
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Further Information
Protein Sequence on NCBI
Material and Safety Data Sheet
Handbook Actin-Toolkit Microinjection
(Web version only for demonstration purpose!)
A full print version with protocols come with the kit.
Flyer Actin-Toolkits
Material and Safety Data Sheet
Protein Sequence on NCBI