Alpha Skeletal Muscle Actin

Novel Kinetic and Affinity Analysis of F-Actin Ligands.


The Actin-Toolkit SPR is a unique method to analyze ligand interactions with actin filaments. The kit is available with α-actin (rabbit skeletal muscle) and bovine α-cardiac actin. The kit is used to analyze kinetics and dissociation constants of ligands binding to F-actin, as well as actin filament bundling or branching induced . 



The analysis of pharmacological compounds, proteins or lipids altering ligand-F-actin interaction, can be analyzed by user-specific methods (e.g. various types of spectroscopy, NMR, EPR, ) or by high speed centrifugation followed by SDS-PAGE analysis and/or immunoblot analysis.