Actin-Toolkit SPR
Native bovine cardiac α-actin
Cat. #: 8900-01
Applications
- Characterization of binding kinetics and dissociation constants of ligands interacting with F-actin.
- Analysis of proteins, lipids or pharmacological compounds that alter ligand–actin interactions.
Description
| Product name | Actin-Toolkit SPR | Bovine Cardiac Actin |
| Protein | Actin (cardiac α-actin) |
| Origin | Bovine cardiac muscle |
| Molecular mass | 42 kDa |
| Toolkit description |
The Actin-Toolkit SPR is designed for analysis of ligand interactions with filamentous cardiac actin by surface plasmon resonance (SPR). The assay format supports characterization of binding kinetics and dissociation constants for proteins, protein fragments and other actin-interacting ligands. To reduce problems associated with long actin filaments in SPR fluidics, the toolkit uses short F-actin filaments anchored through AnkerproteinTM at the fast-growing barbed end. The anchored filaments protrude from the streptavidin-coated sensor surface and remain accessible to analytes introduced during the binding experiment. The system can be used to investigate direct F-actin binding as well as the effects of pharmacological compounds, proteins or lipids on ligand–actin complexes. The toolkit contains native bovine cardiac actin, AnkerproteinTM, FAB-EP and GAB-EP SPR buffers and a dedicated handbook. Streptavidin-coated sensor chips are required and are not included. Experimental conditions for the analyte or compound being investigated must be optimized individually. |
| Purity | >99% by scanning densitometry. |
Properties
| Form | Lyophilized protein components and liquid SPR buffers as listed below. |
| Kit content | 4 × 250 µg Actin (lyophilized powder); 4 × 10 µg AnkerproteinTM (lyophilized powder); 1 × PolyMix (actin polymerization buffer, 10×); 2 × 250 ml FAB-EP, liquid, ready-to-use; 1 × 250 ml GAB-EP, liquid, ready-to-use; 1 × Toolkit Handbook SPR. |
| Buffers | FAB-EP: 100 mM imidazole-Cl, pH 7.4, 0.4 mM ATP, 2 mM CaCl2 and 0.0005% v/v Surfactant P20. GAB-EP: 2 mM Tris-Cl, pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2 and 0.0005% v/v Surfactant P20. |
| Related products | For dilution of G-actin or exchange of ligand buffer into an actin-compatible buffer, MonoMix (Cat. #: 5100-0*) may be used. In the absence of nucleators, polymerization of G-actin can be initiated with PolyMix (Cat. #: 5000-0*). |
| Purification notes | Purified from bovine cardiac muscle; GPC. |
| Protein concentration | Actin concentration determined by direct UV absorbance at 290 nm; A290 nm, 1% = 0.62. |
| Storage instructions | Store at −70 °C upon arrival. Kit components are stable for 6 months under these conditions. Solubilized G-actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | Shipped at ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for human or veterinary diagnostic or therapeutic applications. |
BiacoreTM is a trademark of Cytiva. AnkerproteinTM is a trademark of Hypermol.
Further Information
Handbook Actin-Toolkit SPR
(Web version only for demonstration purpose!)
A full print version with protocols come with the kit.
Flyer Actin-Toolkits
Material and Safety Data Sheet
Protein Sequence on NCBI
