CellStaining Kits - Cytoskeleton

Cell staining • Actin visualization • multiplex-friendly

Visualizing the actin cytoskeleton

Achieve fast, high-contrast labeling of F-actin using Hypermol® Cellstaining Kits and ActinStain reagents. These tools are designed for fluorescence microscopy workflows where brightness, photostability and clean signal are critical.

 
Hypermol® staining reagents
 
Ready-to-use
Designed to reduce setup friction: stable fluorescence signal and clean imaging output for routine staining workflows.
 
Multiplex-friendly
Suitable for double or multi-color experiments when combined with other fluorescent probes and labels.

Cellstaining Kits and ActinStain for actin cytoskeleton visualization

Use these reagents to highlight actin filament organization in fixed or permeabilized cells. Their fluorescence performance supports imaging where background and photostability impact interpretability. For exact handling instructions, staining conditions, and compatibility notes, follow the product page and datasheet for the specific item.

Key features
  • High-contrast actin filament visualization for microscopy.
  • Signal stability designed for extended imaging sessions.
  • Designed for integration into multi-label staining panels.
  • Practical format aimed at fast, reproducible workflows.
Common applications
  • Actin architecture and morphology readouts.
  • Multi-color fluorescence microscopy panels.
  • Cell shape and cytoskeletal rearrangement studies.
  • High-resolution imaging workflows (system-dependent).
 
Available products (examples)
Cellstaining Kit 488 • Cellstaining Kit 647 • Cellstaining Kit 700 • ActinStain 488 • ActinStain 390
 
Browse staining kits & ActinStain
 
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Validated product quality with Satisfaction Guarantee.
 
cell-staining-kit-425
CellStaining Kit 425
λex 439nm, λem 485nm

Rapid staining of single actin filaments with 425-Phalloidin. Suited for cultured cells and cell extracts.
185,00 EUR
excl. Shipping costs
cell-staining-kit-465
CellStaining Kit 465
λex 453nm, λem 506nm

Rapid staining of single actin filaments with 465-Phalloidin. Suited for cultured cells and cell extracts.
185,00 EUR
excl. Shipping costs
cell-staining-kit-488
CellStaining Kit 488
λex 501nm, λem 523nm

Rapid staining of actin filaments with highly photostable 488-Phalloidin. Suited for cultured cells and cell extracts.
185,00 EUR
excl. Shipping costs
cell-staining-kit-647
CellStaining Kit 647
λex 647nm, λem 669nm

Rapid staining of actin filaments with highly photostable 647-Phalloidin. Suited for cultured cells and cell extracts.
185,00 EUR
excl. Shipping costs
cell-staining-kit-700
CellStaining Kit 700
λex 647nm, λem 669nm

Rapid staining of actin filaments with highly photostable 700 phalloidin. Suited for cultured cells and cell extracts.
185,00 EUR
excl. Shipping costs
CellStaining Kit 490LS
CellStaining Kit 490LS
λex 496nm, λem 661nm

Rapid staining of actin filaments with highly photostable 490LS-Phalloidin. Suited for cultured cells and cell extracts.
191,00 EUR
excl. Shipping costs
cell-staining-kit-550
CellStaining Kit 565
λex 564nm, λem 590nm

Rapid staining of single actin filaments with 565-Phalloidin. Suited for cultured cells and cell extracts.
191,00 EUR
excl. Shipping costs
cell-staining-kit-594
CellStaining Kit 594
λex 601nm, λem 627nm
 
Rapid staining of single actin filaments with highly photostable 594-Phalloidin. Suited for cultured cells and cell extracts.
191,00 EUR
excl. Shipping costs
cell-staining-rhodamine
CellStaining Kit Rhodamine
λex 540nm, λem 565nm

The CellStaining kit provides intense, photostable fluorescence for crisp, high-contrast visualization of the actin cytoskeleton and consistent imaging results. Rapid staining of single actin filaments with Rhodamine Phalloidin. 
302,00 EUR
excl. Shipping costs
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Cellstaining Kits and ActinStain for F-actin visualization

Cellstaining Kits and ActinStain reagents are designed for reliable visualization of the actin cytoskeleton (F-actin) in fluorescence microscopy. The portfolio supports routine imaging as well as high-resolution workflows where stable signal and clean contrast matter for interpretation and documentation.

What these reagents are used for
  • F-actin staining in fixed or permeabilized cells for microscopy documentation and analysis
  • Multicolor panels (actin + additional markers) with attention to spectral separation
  • Morphology and structural readouts (stress fibers, cortex, bundles) in cell biology workflows

Key performance points

  • Photostable fluorescence
    Designed for stable imaging signals during acquisition series and documentation.
  • Multiplex-oriented design
    Multiple color options to support clean channel planning and reduced spectral overlap.
  • Microscopy compatibility
    Suitable for standard widefield as well as confocal and high-resolution imaging setups.
  • Workflow clarity
    Focus on practical usability for consistent staining results across runs.

Color selection (with common microscopy channels)

Choose the actin stain channel based on your existing labels and available lasers/filters. The ranges below reflect common microscope channels (typical excitation lines and emission windows), which can help you plan clean spectral separation.

  • Blue (typ. ex 405 nm)
    Useful when green/red channels are reserved for antibodies or fluorescent proteins.
  • Green (typ. ex 488 nm; em ~500–550 nm)
    Standard channel for strong signal; avoid if you run GFP/FITC in the same panel.
  • Red (typ. ex 561 nm; em ~570–620 nm)
    Good choice for multiplexing when the green channel is occupied.
  • Far-Red (typ. ex 633/640 nm; em ~650–750 nm)
    Often helpful to reduce spectral crowding; commonly used in multicolor panels.
Note: Exact excitation/emission maxima depend on the specific dye used. Please refer to the individual product page for the documented optical data.
Need help selecting the best match?
Send us your microscope configuration (lasers/filters) and your panel (e.g., DAPI / GFP / TRITC / Cy5). We can suggest a compatible actin stain.
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