CellStaining Kit 565
Suited for cultured cells & cell extracts
Cat. #: C8813-01
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Applications
Rapid staining of actin filaments with highly photostable 565-Phalloidin. Suited for cultured cells & cell extracts.
Replacent for: Alexa Fluor® 555, Cy™3,Tetramethylrhodamine.
Description
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Product
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565-Phalloidin (ATTO565 phalloidin)
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Origin
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Amanita Phalloides Conjugate
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Molecular mass
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N/A
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Product description
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Rapid staining of single actin filaments with highly photostable 565-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments.
Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin.
565-Phalloidin has a strong orange fluorescence of high brightness and superior photostability.
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Actin interaction
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Selective staining of F-actin in fixed and permeabilized cells and cell free F-actin.
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Description
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Form
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Dry
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Quantity
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1x 100 Units (sufficient for 100 slides)
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Buffer
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Reconstitute in 50µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain the sample.
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Sub cellular localization
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Actin, Cytoskeleton
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Excitation/Emission
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ex 564 nm, em 590 nm
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Kit Content
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1x 100 Units 565-Phalloidin
1x PBS tablet to make 100ml PBS
1.0 ml FluoProtect
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Storage instructions
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Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.
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Shipping conditions
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At ambient temperature. Upon delivery store at -5 to -70°C.
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Remarks
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CAS no.
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N/A
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Further Information
Table of Dyes and Light Sources