CellStaining Kit 425

Product

425-Phalloidin (ATTO425 phalloidin)

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid staining of single actin filaments with highly photostable 425-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments.

Labelled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 

425-Phalloidin has a strong pink fluorescence of high brightness and superior photostability.

Actin interaction

Selective staining of F-actin in fixed and permeabilized cells and cell free F-actin.

Form

Dry

Quantity

1x 100 Units (sufficient for 100 slides)

Buffer

Reconstitute in 50µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain  the sample.

Sub cellular localization

Actin, Cytoskeleton

Excitation/Emission

ex 439 nm, em 485 nm

Kit Content

1x 100 Units 425-Phalloidin
1x PBS tablet to make 100ml PBS 
1.0 ml FluoProtect 

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions 

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

CAS no.

 N/A