Goat Anti-Mouse IgM (H+L) ATTO430LS
Antibody produced in goat
Cat. # 2203-1MG
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Description
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Product name |
Goat Anti-Mouse IgM ATTO430LS | GXM IgM ATTO430 |
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Target species |
Mouse |
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Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, distinguished by their exceptional fluorescence intensity, superior photostability, and reliability in diverse applications. Goat Anti-Mouse IgM ATTO430LS is notable for its large Stokes shift of 109 nm, providing excellent separation between excitation and emission wavelengths. This feature makes it particularly well-suited for multiplexing approaches, enabling precise signal discrimination when used alongside fluorophores with overlapping spectra. The antibody is preservative-free, making it compatible with a wide range of applications, including live-cell imaging and other sensitive techniques. Its strong fluorescence signal, minimal non-specific binding, and enhanced photostability ensure high performance in demanding experimental conditions, such as complex tissue samples or dynamic live-cell environments. |
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Specification |
Goat Anti-Mouse IgM ATTO430LS (H+L), Immunofluorescence: 1:500-1:1500, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence. |
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Optical Properties |
λex: 436nm, λem: 545nm |
Properties
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Form |
Lyophilized |
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Nominal concentration |
2 mg/ml |
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Content
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1 mg Goat Anti-Mouse IgM (H+L) ATTO430LS |
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Clonality |
Polyclonal |
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Isotype |
IgM |
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Purification notes |
Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. |
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Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles. |
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Shipping conditions |
Shipped at ambient temperature. |
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Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
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CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary AntibodiesSuper-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016 Mar 11;7:10917. doi: 10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
