Goat Anti-Mouse IgM (H+L) ATTO430LS
Antibody produced in goat, affinity purified
Cat. #: 2203-1MG
Applications
Fluorescent secondary antibody for detection of mouse IgM in immunofluorescence microscopy and related fluorescence-based assays. The large Stokes shift of ATTO430LS can be advantageous for spectral separation in multiplex fluorescence workflows.
Description
| Product name | Goat Anti-Mouse IgM (H+L) ATTO430LS | GXM IgM ATTO430LS |
| Target species | Mouse |
| Description |
Goat Anti-Mouse IgM (H+L) ATTO430LS is an antigen-specific fluorescent secondary antibody for detection of mouse IgM. Affinity purification removes essentially all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. The antibody is conjugated to ATTO430LS and further purified by gel filtration to remove unbound dye and low-molecular-weight reaction components. ATTO430LS has an absorption maximum at 436 nm and a fluorescence maximum at 545 nm, corresponding to a Stokes shift of 109 nm. The antibody is supplied as a lyophilized preparation and can be reconstituted to a 2 mg/ml stock solution. Protect the fluorescent conjugate from light. |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 436 nm, λfl: 545 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml after reconstitution with 0.5 ml glycerol buffer. |
| Content | 1.0 mg Goat Anti-Mouse IgM (H+L) ATTO430LS 20 µg monoclonal anti-actin reference antibody (1 mg/ml) 1.0 ml glycerol buffer supplied; add 0.5 ml to reconstitute the lyophilized secondary antibody. Final buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Target immunoglobulin | Mouse IgM (H+L) |
| Purification notes | Antigen-specific affinity purification removes essentially all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. Following conjugation to ATTO430LS, the antibody is further purified by gel filtration. |
| Storage instructions | After reconstitution, store at 2–8 °C for up to three months. For extended storage, aliquot and store at −20 °C; avoid repeated freeze/thaw cycles. Protect the fluorescent conjugate from light. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017;544(7651):465-470. doi:10.1038/nature22051.
αv Integrins combine with LC3 and atg5 to regulate Toll-like receptor signalling in B cells.
Acharya M, Sokolovska A, Tam JM, Conway KL, Stefani C, Raso F, Mukhopadhyay S, Feliu M, Paul E, Savill J, Hynes RO, Xavier RJ, Vyas JM, Stuart LM, Lacy-Hulbert A.
Nat Commun. 2016;7:10917. doi:10.1038/ncomms10917.
Mechanical signaling coordinates the embryonic heartbeat.
Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ.
Proc Natl Acad Sci U S A. 2016;113(32):8939-8944. doi:10.1073/pnas.1520428113.
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