
Goat Anti-Rat IgG ATTO390 (H+L)
Antibody produced in goat, affinity purified
Cat. #: 2801-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
Product name |
Goat Anti-Rat IgG ATTO390 (H+L) | GXRt ATTO390 |
Target species |
Rat |
Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Rat IgG ATTO390 (H+L) is specifically optimized for high-resolution microscopy, offering remarkable photostability and a high fluorescence quantum yield. These properties enable prolonged imaging sessions with minimal photobleaching, ensuring sharp and reliable signals.
ATTO390 exhibits a distinct excitation/emission spectrum (λex: 390 nm, λem: 479 nm) with a long Stokes shift, providing excellent compatibility with UV-excitable systems and minimizing spectral overlap in multiplex imaging setups. Its strong fluorescence signal and low autofluorescence background make it highly effective in detecting low-abundance targets and achieving precise spatial resolution, even in dense or complex biological samples.
This antibody is preservative-free, making it suitable for live-cell imaging and other sensitive applications requiring minimal interference. Its low non-specific binding and high affinity ensure accurate target detection, while its robust fluorescence properties allow consistent results in advanced techniques such as super-resolution microscopy, flow cytometry, and fluorescence resonance energy transfer (FRET) assays.
|
Specification |
Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
Optical Properties |
λex: 390 nm, λem: 479 nm |
Properties
Form |
Lyophilized |
Nominal concentration |
2 mg/ml (after reconstitution with buffer) |
Content |
1.0 mg Goat Anti-Rat IgG ATTO390 (H+L)
1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody)
Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
Clonality |
Polyclonal |
Isotype |
IgG |
Purification notes |
Column affinity purified |
Storage instructions |
Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
Shipping conditions |
Shipped at ambient temperature. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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