Goat Anti-Rat IgG ATTO700 (H+L)
Antibody produced in goat, affinity purified
Cat. #: 2811-1MG
Applications
Suited for microscopy and live-cell techniques, containing no preservatives for compatibility with sensitive applications.
Description
| Product name | Goat Anti-Rat IgG ATTO700 (H+L) | GXRt ATTO700 |
| Target species | Rat |
| Description |
ATTO-labeled secondary antibodies represent a new generation of fluorescent reagents, characterized by their exceptional fluorescence intensity, superior photostability, and consistent performance across various applications. Goat Anti-Rat IgG ATTO700 (H+L) is optimized for high-performance imaging, offering robust fluorescence signals and excellent stability for extended experimental workflows. The ATTO700 dye features a distinct excitation/emission spectrum (λex: 700 nm, λem: 720 nm), ideal for near-infrared imaging applications. Its long-wavelength properties minimize autofluorescence and enhance imaging depth, making it highly effective for thick tissue samples and live-cell imaging in complex biological environments. This antibody is preservative-free, ensuring compatibility with live-cell imaging and other sensitive applications. Its low non-specific binding and high affinity provide accurate and reliable target detection, while its superior fluorescence properties facilitate advanced techniques such as super-resolution microscopy, flow cytometry, and fluorescence lifetime imaging microscopy (FLIM). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-6, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 700 nm, λem: 720 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Rat IgG ATTO700 (H+L) 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to rat IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
References
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Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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