Protein

Thymosin beta-4 (Synonyms: T beta 4, Fx , TB4X, PTMB4, TMSB4)

Origin

recombinant, human recombinant - untagged.

Molecular mass

5kDa

Protein description

Thymosin is a hormone secreted from the thymus. The predominant form of thymosin, thymosin b4, is a member of a highly conserved family of actin monomer sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin bound pool and F-actin.

Actin interaction

The predominant form of thymosin, thymosin b4, is a member of a highly conserved family of actin monomer sequestering proteins. b-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin bound pool and F-actin.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x50µg

Buffer

5mM Tris pH 8.1, 0.1mM DTT and 0.2% sucrose, when reconstituted with 50µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity 

>98% by RT-HPLC.

Purification notes

Thymosin beta-4 protein was expressed in E. coli and further purified by Affinity Chromatography (Polyproline column). The pure protein was yielded by gel filtration. 

Protein concentration

Determined by by RT-HPLC.

Storage instructions

Profilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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