Protein

Tropomyosin-Troponin (Synonym: native Tropomyosin) 

Origin

Skeletal muscle, rabbit

Molecular mass

~148kDa

Molecular structure

Tropomyosin (2x35kDa), Troponin T (37kDa),Troponin I (23kDa),Troponin C (18kDa).   

Actin interaction

Ca++-sensitive, regulatory laterally F-actin binding complex. Molar ratio = 1:7 (tropomyosin/troponin:g-actin).

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

0.1M KCl, 20mM imidazole pH 7.4, 1mM EDTA, 2mM DTT and 2% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution.

Purity

>95% by scanning densitometry.

Purification notes

Purified from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Tropomyosin/Troponin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized complex is kept on ice and should be used within 1 week.

This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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