Protein

Pyrene Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. Pyrene-Actin is chemically modified G-actin, carrying a pyrenyl group covalently bound to Cys374 at the C-term of the molecule.

The actin polymerization can be induced simply by mono- or divalent cations (in-vitro conditions) and the actin kinetic can then be measured fluorometrically. Fluorometry with Pyrenyl-Actin is standard  method  to study the effect of ligands on actin kinetics.

Conjugation ratio

Pyrene Actin (10%) contains 10% of pyrenyl actin with a dye/protein molar ratio of 1. 

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Pyrene actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.