Protein

NEM-Myosin

Origin

Skeletal muscle, rabbit

Molecular mass

~480kDa

Protein description

NEM-Myosin II is chemically inactivated fast muscle myosin II from rabbit psoas muscle. Myosin II is a hexameric, actin-binding ATPase with a total molecular weight of 480kD in a head- and-tail conformation.

Myosin II has two identical head domains, where the actin binding sites are localized. It consists of two heavy chains (each 220kD) and four light chains (ELC=essential and RLC= regulatory light chains). 

Actin interaction

Binds to F-actin.

Form

Lyophilized, ready-to-use.

Quantity

1x200 µg

Buffer

500mM KCl, 20mM Imidazole pH 7.0, 2mM DTT and 8% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Purified from rabbit skeletal muscle (m. psoas).

Protein concentration

Determined at OD280 (0.1% = 0.53).

Storage instructions

Upon reconstitution the product is stored on ice. This product can also be stored as a glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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