Myosin S1 (from rabbit m. psoas) - 2x100µg
Myosin S1
Actin-binding protein fragment.
Applications
| Applications | Motility assays, actin-based assays and biochemical assays. |
Description
| Protein | Myosin S1, fragment of myosin II from m. psoas |
| Origin | Skeletal muscle, rabbit |
| Molecular mass | 110 kDa |
| Protein description | Myosin S1 is obtained by limited papain cleavage of myosin II heavy meromyosin (HMM) from rabbit psoas muscle. The fragment has a molecular mass of approximately 110 kDa. Myosin S1 represents the motor domain of myosin II and contains the actin-binding and ATP-binding sites. It is therefore well suited for biochemical assays addressing actomyosin interaction, ATPase activity and actin-dependent motor function. |
| Actin interaction | Binds to F-actin. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 2 × 100 µg |
| Buffer | 50 mM KCl, 10 mM Tris pH 7.6, 0.2 mM DTT and 1% disaccharides, when reconstituted with 100 µl ultrapure water. |
| Purity | >97% by scanning densitometry from Coomassie G-250 stained SDS gels. |
| Purification notes | Purified from rabbit skeletal muscle, m. psoas. |
| Protein concentration | Determined by UV absorbance: 1 mg/ml corresponds to A275 = 0.75. |
| Storage instructions | Upon reconstitution, store the product on ice. The product can also be stored as a glycerol stock at −20 °C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
