ADP | 100mM, pH 7.4 - 2x 250µl
ADP
100 mM, pH 7.4 | 2x 250 µl
Applications
Description
| Product name | ADP, Synonyms: Adenosine 5'-diphosphate |
| Product description | Adenosine 5'-diphosphate (ADP) is a central nucleotide in cellular energy metabolism and a key ligand for ATPase-driven protein systems. In actin- and myosin-based assays, ADP is used to probe nucleotide-dependent conformational states and kinetics. The supplied ADP is pre-adjusted to pH 7.4 for stability and convenience. Reconstitution with 250 µl ultrapure water yields a 100 mM stock solution with consistent pH, avoiding additional titration steps. |
| Overview | Suited to supplement all buffers and solutions buffered at ~pH 7.4. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity | Powder: 2x 100 mM ADP, 20 mM Hepes titrated with NaOH to pH 7.4. Sterile filtered prior to freeze-drying. Weigh directly into the tube 250 mg of ultrapure H2O, corresponding to 250 µl ultrapure H2O, to obtain a fresh 100 mM stock solution. |
| Formula | Empirical Formula (Hill Notation) C10H15N5O10P2 (free acid); Molecular Weight 427.20 of the free acid without buffer. |
| Purity | ADP ≥ 95% (HPLC). Ultrapure buffer reagents. |
| Storage instructions | ADP is stored at −20 °C upon arrival. The product will be stable in performance for at least 1 year from the date of purchase. Solubilized ADP can be aliquoted and stored at −70 °C for use within 3 months. Thawed aliquots should be used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −20 °C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet