
FAB-P
10x F-Actin buffer with Surfactant P-20 for SPR (liquid)
Cat. #: 5220-01
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Description
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Product name
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FAB-P
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Product description
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FAB-P is used for F-Actin assays in SPR techniques to maintain the structural integrity of actin filaments, for dilution and preparation of actin and ligand samples. The buffer contains all components essential for the biological activity of actin. FAB-P is a sterile filtered and degased 10x solution. Upon addition of ATP (included as a separate component) the buffer is adjusted to pH 7.4 and finally degased (unless an in-line degasser is installed).
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Overview
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FAB-P is used to:
- support ligand binding to F-actin;
- maintain the filamentous state of actin;
- prevent denaturing of actin;
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Properties
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Form
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Liquid, ready-to-use.
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Quantity
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1x25 ml (10x solution to make 250 ml)
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Buffer
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1M KCl, 0.4mM ATP, 2mM MgCl2., 0.0005% Surfactant P20, Buffer: 10mM midazole pH 7.4.
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Purity
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Ultrapure reagents in Milli-Q™
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Storage instructions
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FAB-P is stored at -20°C upon arrival and will be stable in performance for 3 months from the date of shipping. Prepare aliquots to store at -20°C and avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -20°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Buffers from Hypermol are made of purest reagents in Milli-Q™
water, as described in our publications.
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