Actin-Toolkit Crystallography
Actin-Toolkit Crystallography
Non-Polymerizing Actin (enzymatically modified rabbit skeletal muscle α-Actin)
Cat. #: 8050-01
Applications
Description
Protein |
Non-Polymerizing Actin |
Origin |
Skeletal muscle alpha actin, rabbit |
Molecular mass |
42kDa |
Toolkit description |
Non-Polymerizing Actin is alpha skeletal muscle actin, enzymatically cleaved between Gly42/Val43. As a result of this cleavage an essential monomer-monomer interaction site is inactivated, rendering the actin unpolymerizable in the presence of Ca++, while it is fully polymerizable in the presence of Mg++. Also in the presence of e.g. 100mM KCl, NP-Actin does not polymerize unless 2mM MgCl2 are added to the solution. Crystallization of actin binding proteins with G-actin is complicated by the polymerization of actin. An advantage for crystallization of actin and co-crystallization of ligand-actin complexes is the use of Non-Polymerizing Actin. The Toolkit is furthermore used for biophysical methods investigating conformational changes upon complex formation in actin or the actin ligand. Without chemical modification of actin, Non-Polymerizing Actin can be used for crystallography, biophysical or biochemical analysis. The ability of NP-Actin to bind to other proteins remains essentially unimpaired. For initial tests, we offer the Actin-Trialkit Crystallography (Cat. # T8040-01). The Actin-Toolkit Crystallography contains 6x 1 mg Non-Polymerizing Actin prepared from purified rabbit skeletal muscle actin. A handbook with a detailed description for a safe, error-free handling of NP-Actin is provided with the kit. |
Purity |
>99% by scanning densitometry. |
Properties
Form |
Lyophilized, ready-to-use. |
Kit content |
6x 1.0 mg NP-Actin (lyophilized powder); |
Actin Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100µl ultrapure water to obtain a 1mg/ml solution. |
DOL |
- |
Purification notes |
Purified from rabbit skeletal muscle, GPC. |
Protein concentration |
Determined by the Biuret method. |
Storage instructions |
We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing. |
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
CAS no. |
Further Information
Material and Safety Data Sheet
Protein Sequence on NCBI
Protein Sequences and Structures on RCSB
Literature
Material and Safety Data Sheet
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