Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.
Actin interaction
Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio.
Properties
Form
Lyophilized, ready-to-use.
Quantity per unit
1x1 mg
Buffer
150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution.
Purity
>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Purification notes
Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.
Protein concentration
Determined by OD280 (0.1%=0.778)
Storage instructions
Cofilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.
Shipping conditions
At ambient temperature. Upon delivery store at -70°C.
Remarks
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.