Protein

Cofilin (synonym: n-cofilin , cofilin-1)

Origin

non-muscle isoform, murine recombinant (untagged)

Molecular mass

18.5kDa

Protein description

Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.

Actin interaction

Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio. 

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution.

Purity

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Cofilin was  expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. 

Protein concentration

Determined by OD280 (0.1%=0.778)

Storage instructions

Cofilin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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