Vinculin
(Actin Binding Protein)
| Protein | Vinculin |
| Origin | Smooth muscle, turkey |
| Molecular mass | 116 kDa |
| Protein description | Vinculin from turkey smooth muscle (gizzard) is a single chain polypeptide with a molecular weight of 116 kDa. Vinculin is a multiligand binding protein (e.g. of focal contacts), possessing an F-actin binding domain in the tail region. Vinculin is supplied in the native inactive state, characterized by the interaction of the vinculin head and tail, which can be released e.g. by phospholipids. |
| Actin interaction | Actin binding protein. |
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 1 × 250 µg |
| Buffer | 100 mM KCl, 10 mM imidazole pH 7.5, 0.5 mM DTT, 0.1 mM EGTA and 0.25% disaccharides, when reconstituted with 250 µl ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
| Purification notes | Purified from turkey gizzard muscle. |
| Protein concentration | Determined by Biuret method. |
| Storage instructions | Vinculin is stored at −70 °C upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 2-3 weeks. This product can also be stored as a glycerol stock at −20 °C. Avoid repeated freeze / thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Reconstitution of contractile actomyosin rings in vesicles.
Litschel T, Kelley CF, Holz D, Adeli Koudehi M, Vogel SK, Burbaum L, Mizuno N, Vavylonis D, Schwille P.
Nat Commun. 2021 Apr 15;12(1):2254. doi: 10.1038/s41467-021-22422-7.
Phosphoinositides regulate force-independent interactions between talin, vinculin, and actin.
Kelley CF, Litschel T, Schumacher S, Dedden D, Schwille P, Mizuno N.
eLife. 2020 Jul 13;9:e56110. doi: 10.7554/eLife.56110.
Binding of Vinculin to Lipid Membranes in Its Inhibited and Activated States.
Dwivedi M, Winter R.
Biophys J. 2016 Oct 4;111(7):1444-1453. doi: 10.1016/j.bpj.2016.08.019.
Structural basis for vinculin activation at sites of cell adhesion.
Bakolitsa C, Cohen DM, Bankston LA, Bobkov AA, Cadwell GW, Jennings L, Critchley DR, Craig SW, Liddington RC.
Nature. 2004 Jul 29;430(6999):583-6. Epub 2004 Jun 13.
