Biotin-Actin (alpha-cardiac actin, bovine cardiac muscle) 2x100µg

Protein

Biotin-Actin

Origin

Cardiac muscle alpha actin, bovine

Molecular mass

43kDa

Protein description

Biotin-Actin from cardiac muscle is chemically modified α-actin by esterification of lysine residues with biotin. The advantage of Biotin-Actin from Hypermol is, that it is equipped  with a ~20 Å spacer, which renders the Biotin group to be readily available for antibody detection in  all suitable techniques (ELISA, Western Blotting etc.)..  

Conjugation ratio

The degree of labelling (DOL) of Biotin-Actin is approx. 1.

Form

Lyophilized, ready-to-use.

Quantity per unit

2x100 µg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC from bovine cardiac muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

Biotin-Actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be used within 1 week. Avoid refreezing. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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