NBD-Actin - 1.0mg

Protein

NBD-Actin

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

43kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. NBD-Actin is chemically modified G-actin.

The first modification step results in NEM-actin (N-ethylmaleimide actin). Subsequent covalent conjugation of 4-Chloro-7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) to Lys372 yields green fluorescent NBD-Actin.

Optical properties: Abs. max. 337nm; Em. max. 512nm

Conjugation ratio

DOL=0.8. 

Form

Lyophilized, ready-to-use.

Quantity per unit

1x1 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified from rabbit skeletal muscle.

Protein concentration

Determined by the Biuret method.

Storage instructions

NBD-actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

CAS no.