NBD-Actin
alpha actin, skeletal muscle rabbit
Cat. #: 8107-01
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Applications
Fluorimetrical Analysis of actin dynamics (e.g. kinetics of polymerization, nucleation, depolymerization)
Description
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Protein
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NBD-Actin
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Origin
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Skeletal muscle alpha actin, rabbit
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Molecular mass
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43kDa
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Protein description
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G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. NBD-Actin is chemically modified G-actin.
The first modification step results in NEM-actin (N-ethylmaleimide actin). Subsequent covalent conjugation of 4-Chloro-7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) to Lys372 yields green fluorescent NBD-Actin.
Optical properties: Abs. max. 337nm; Em. max. 512nm
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Conjugation ratio
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DOL=0.8.
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Properties
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Form
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Lyophilized, ready-to-use.
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Quantity per unit
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1x1 mg
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Buffer
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2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution.
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Purity
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>99% by scanning densitometry.
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Purification notes
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Purified from rabbit skeletal muscle.
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Protein concentration
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Determined by the Biuret method.
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Storage instructions
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NBD-actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized pyrene actin is kept on ice and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Structural basis of actin monomer re-charging by cyclase-associated protein.
Kotila T, Kogan K, Enkavi G, Guo S, Vattulainen I, Goode BL, Lappalainen P.Nat Commun. 2018 May 14;9(1):1892. doi: 10.1038/s41467-018-04231-7.PMID: 29760438