NBD-Actin
α-skeletal muscle actin, rabbit
Cat. #: 8107-01
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Description
|
Protein |
NBD-Actin |
|
Origin |
Skeletal muscle α-actin, rabbit |
|
Molecular mass |
43kDa |
|
Protein description |
G-actin from rabbit skeletal muscle is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. NBD-Actin is a two-step chemically modified G-actin. The first modification step results in NEM-actin (N-ethylmaleimide actin). Subsequent covalent conjugation of 4-Chloro-7-Nitrobenz-2-Oxa-1,3-Diazole (NBD) to Lys372 yields green fluorescent NBD-Actin. Optical properties: λex337nm, λem 512nm |
|
Conjugation ratio |
DOL=0.8. |
Properties
|
Form |
Lyophilized, ready-to-use. |
|
Quantity per unit |
1 mg |
|
Buffer |
2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1 ml ultrapure water to obtain a 1mg/ml solution. |
|
Purity |
>99% by scanning densitometry. |
|
Purification notes |
Purified from rabbit skeletal muscle. |
|
Protein concentration |
Determined by the Biuret method. |
|
Storage instructions |
NBD-actin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized NBD actin is kept on ice and should be used within 1 week. |
|
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
|
Remarks |
|
|
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
Structural basis of actin monomer re-charging by cyclase-associated protein.
Kotila T, Kogan K, Enkavi G, Guo S, Vattulainen I, Goode BL, Lappalainen P.Nat Commun. 2018 May 14;9(1):1892. doi: 10.1038/s41467-018-04231-7.PMID: 29760438
Product DataSheetMaterial and Safety Data SheetProtein Sequence on NCBI
Structural basis of actin monomer re-charging by cyclase-associated protein.
Kotila T, Kogan K, Enkavi G, Guo S, Vattulainen I, Goode BL, Lappalainen P.Nat Commun. 2018 May 14;9(1):1892. doi: 10.1038/s41467-018-04231-7.PMID: 29760438
