Ezrin (human, recombinant) - 2x50 µg
Ezrin
(Actin Binding Protein)
Description
| Protein | Ezrin (synonym: villin-2, cytovillin) |
| Origin | Human, recombinant (untagged) |
| Molecular mass | 70 kDa |
| Protein description | Ezrin is probably participating in connecting the cytoskeleton to the plasma membrane. Like many proteins, ezrin is autoinhibited by a head-tail interaction. |
| Actin interaction | The C-ter binds to actin, PIP2 and membrane proteins, e.g. CD44. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 2 × 50 µg |
| Buffer | 100 mM KCl, 20 mM Hepes pH 7.4, 0.2 mM DTT and 5% sucrose, when reconstituted with 50 µl ultrapure water to obtain a 1.0 mg/ml solution. |
| Purity | >96% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
| Purification notes | Ezrin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. |
| Protein concentration | Determined by OD280 (0.1% = 0.963) |
| Storage instructions | Ezrin stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at −20 °C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Disorazoles Block Group A Streptococcal Invasion into Epithelial Cells Via Interference with the Host Factor Ezrin.
Rox K, Rohde M, Chhatwal GS, Müller R.
Cell Chem Biol. 2017 Feb 16;24(2):159-170. doi: 10.1016/j.chembiol.2016.12.011. Epub 2017 Jan 12.
Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor.
Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ.
J Biol Chem. 2004 Feb 6;279(6):4840-8. Epub 2003 Nov 18.
