Ezrin (human, recombinant) - 250 µg

Protein

Ezrin (synonym: villin-2, cytovillin)

Origin

human, recombinant (untagged)

Molecular mass

70kDa

Protein description

Ezrin is probably participating in connecting the cytoskeleton to the plasma membrane. Like many proteins, Ezrin is autoinhibited by a head-tail interaction.

Actin interaction

The C-ter binds to actin, PIP2 and membrane proteins, e.g. CD44. 

Form

Lyophilized, ready-to-use.

Quantity per unit

1x250 µg

Buffer

100mM KCl, 20mM Hepes pH 7.4, 0.2mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution.

Purity

>96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.

Purification notes

Ezrin was  expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. 

Protein concentration

Determined by OD280 (0.1%=0.963)

Storage instructions

Ezrin is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20°C in glycerol or CryoProtect (Cat.#: 6011-01).  Avoid repeated freeze / thaw cycles. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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