
ATTO390-Actin for TIRFM
Non-muscle actin from brain, porcine
Cat. #: 8352-03
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Description
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Protein
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ATTO390-Actin
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Origin
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Non-muscle β/γ actin, porcine
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Molecular mass
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42kDa
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Protein description
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Actin from non-muscle is a mixture of β/γ non-muscle actin. Both actin isoforms are single chain polypeptides with a molecular weight of 42kD consisting of 375 amino acids. ATTO390-Actin is chemically modified G-actin with an ATTO390 fluorescent group covalently bound to lysine residues. ATTO390-Actin (λex 390nm, λem 479nm) possesses the polymerization properties of native G-actin.
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Conjugation ratio
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This product is supplied with a degree of labelling (DOL) of ~0.3.. and is thus ready -to-use for TIRFM and other high- or super-resolution imaging techniques
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Properties
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Form
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Lyophilized, ready-to-use.
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Quantity per unit
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2x50 µg
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Buffer
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2.0mM Tris-Cl pH 8.2, 1.0mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 100 µl ultrapure water to obtain a 1mg/ml solution.
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Purity
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>99% by scanning densitometry..
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Purification notes
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Purified by GPC from porcine brain..
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Protein concentration
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Determined by the Biuret method..
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Storage instructions
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ATTO-Actins are stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin is kept on ice and should be used within 1 week. Avoid refreezing.
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Shipping conditions
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At ambient temperature. Upon delivery store at -70°C.
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Remarks
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For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
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CAS no.
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Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Protein Sequence on NCBI