Etheno-ATP-Actin (alpha skeletal muscle actin) - 500 µg
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Etheno-ATP Actin (ε-ATP-Actin)
skeletal muscle α-actin, rabbit
Applications
- Fluorescence quenching
- Exchange rate from G-actin triggered by actin binding proteins
- RET-studies on conformational changes
- Fluorescence anisotropy changes during protein binding
- Exchange rate from G-actin triggered by actin binding proteins
- RET-studies on conformational changes
- Fluorescence anisotropy changes during protein binding
Description
| Protein | Actin (α-Actin) |
| Origin | Skeletal muscle alpha actin, rabbit |
| Molecular mass | 42 kDa |
| Protein description | G-actin from rabbit skeletal muscle is a single chain polypeptide (42 kDa, 375 aa) with stabilizing binding sites for adenosine phosphate nucleotides and divalent cations. Both are necessary to stabilize the tertiary structure of actin. The fluorescent Etheno-ATP (1, N6 -Etheno-adenosine-5’-triphosphate, sodium salt) actin has the following spectroscopic properties: λmax 275 nm, ε 6.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 300 nm, λem 415 nm. Etheno-ATP Actin is supplied as lyophilized powder containing 2 mM Tris-Cl pH 8.2, 1.0 mM ε-ATP, 0.1 mM DTT, 0.08 mM CaCl2 and 0.2% disaccharides, when reconstituted with 0.5 ml H2O. |
| Purity | >99% by scanning densitometry. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 0.5 mg |
| Buffer | 2 mM Tris-Cl pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2, 1 mM NaN3 and 0.3% disaccharides, when reconstituted with 0.5 ml ultrapure water to obtain a 1 mg/ml solution. |
| Related products | The corresponding buffer (10x Etheno-ATP MonoMix, 500 µl) comes with the G-actin. |
| Purification notes | Purified from rabbit skeletal muscle, GPC. |
| Protein concentration | Determined by the Biuret method. |
| Storage instructions | Avoid refreezing. Etheno-ATP Actin stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 3–4 days. |
| Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | 51005-14-2 |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
The actin/actin interactions involving the N-terminus of the DNase-I-binding loop are crucial for stabilization of the actin filament.
Khaitlina SY, Moraczewska J, Strzelecka-Gołaszewska H. Eur J Biochem. 1993 Dec 15;218(3):911-20.
