Etheno-ATP-Actin (alpha skeletal muscle actin) - 500 µg

Protein

Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Protein description

G-actin from rabbit skeletal muscle is a single chain polypeptide (42kDa, 375 aa) with stabilizing binding sites for adenosine phosphate nucleotides and divalent cations. Both are essentially necessary to stabilize the tertiary structure of actin. The fluorescent Etheno-ATP (1, N6 -Etheno-adenosine-5’-triphosphate, sodium salt) of Actin has the following spectroscopic properties: λmax 275 nm, ε 6.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), λexc 300 nm, λem 415 nm. Ethno-ATP Actin (500µg) is supplied as lyophilized powder containing 2mM Tris-Cl pH 8.2, 1.0mM e-ATP, 0.1mM DTT, 0.08mM CaCl₂ and 0.2% disaccharides, when reconstituted with 0.5ml H₂O.

Purity

>99% by scanning densitometry.

Form

Lyophilized, ready-to-use.

Quantity per unit

0.5 mg

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 0.5 ml ultrapure water to obtain a 1mg/ml solution.

Related products

The corresponding buffer (10x Etheno-ATP MonoMix, 500µl) comes with the G-actin.

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

Avoid refreezing. Etheno-ATP Actin stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized G-actin is kept on ice and should be used within 3-4 days. 

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 51005-14-2