Goat Anti-Rabbit IgG (H+L) ATTO740
Antibody produced in goat, affinity purified
Cat. #: 2311-1MG
Applications
Optimized for far-red fluorescence imaging, including confocal microscopy, flow cytometry, and other fluorescence-based techniques. The antibody contains no preservatives, ensuring compatibility with sensitive assays.
Description
| Product name | Goat Anti-Rabbit IgG (H+L) ATTO740 | GXR ATTO740 |
| Target species | Rabbit |
| Description |
ATTO-labeled secondary antibodies set a benchmark in fluorescence labeling, renowned for their intense brightness, superior photostability, and unmatched reliability in a range of applications. Goat Anti-Rabbit IgG ATTO740 is meticulously optimized for far-red fluorescence imaging, providing a high signal-to-noise ratio in demanding experimental setups. ATTO740 features a distinct excitation/emission spectrum (λex: 740 nm, λem: 770 nm), making it an excellent choice for applications requiring minimal spectral overlap and superior resolution in multiplex imaging. Its long-wavelength properties minimize autofluorescence and enhance imaging depth, making it highly effective for thick tissue samples and live-cell imaging. This antibody is preservative-free, ensuring compatibility with live-cell imaging and other sensitive assays. Its exceptional photostability and low background signal enable consistent performance across techniques such as super-resolution microscopy, flow cytometry, and fluorescence lifetime imaging (FLIM). |
| Specification | Immunofluorescence: 1:500–1:1500, Degree of Labeling (DOL): 2-9, Unconjugated dye ≤5% of total fluorescence. |
| Optical Properties | λex: 740 nm, λem: 770 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml (after reconstitution with buffer) |
| Content | 1.0 mg Goat Anti-Rabbit IgG (H+L) ATTO740 1.0 ml Glycerol buffer (add 0.5 ml to reconstitute the lyophilized antibody) Buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification notes | Affinity purification effectively removed all goat serum proteins, including immunoglobulins not specifically binding to rabbit IgG. After conjugation to the dye, the antibody was further purified by gel filtration, resulting in a highly pure and specific antibody. Hypermol® secondary antibodies are highly specific and purified to remove unbound dye, minimizing background signal. |
| Storage instructions | Store as glycerol stock at -20°C. Avoid repeated freeze/thaw cycles. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
Buffers and antibodies from Hypermol® are made of the purest reagents in Milli-Q™ water, as described in our publications.
Further Information
Material and Safety Data Sheet
References
Super-multiplex vibrational imaging.
Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051.
Physiologic and Nanoscale Distinctions Define Glutamatergic Synapses in Tonic vs Phasic Neurons.
He K, Han Y, Li X, et al.
J Neurosci. 2023;43(25):4598-4611. doi:10.1523/JNEUROSCI.0046-23.2023.
Bitbow Enables Highly Efficient Neuronal Lineage Tracing and Morphology Reconstruction in Single Drosophila Brains.
Li Y, et al.
Front Neural Circuits. 2021;15:732183. doi:10.3389/fncir.2021.732183.
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Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies Product DataSheet
Material and Safety Data SheetTable of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
