ATTO-labeled goat-anti rabbit IgG f(ab) fragments belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Rabbit IgG f(ab)-ATTO488 is specifically suited for high and super resolution microscopy, due to the high photostability and strong fluorescence quantum yield.
Conjugates of f(ab) fragments are preferable to whole antibody conjugates:
- for cellular structures which are sterically difficult to access;
- prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in Fab fragments;
Specification
Goat Anti-Rabbit IgG f(ab) ATTO488, Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence.
Optical properties
λex 501nm, λem 523nm
Properties
Form
Lyophilized
Nominal concentration
2mg/ml
Content
Buffer
0.25 mg Anti-Rabbit IgG (Fab) ATTO488 1.0 ml Glycerol buffer
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4.
Clonality
Polyclonal
Isotype
IgG
Purification notes
Column affinity purified, IEX chromatography after papain cleavage.
Storage instructions
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping conditions
Shipped at ambient temperature.
Remarks
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
CAS no.
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
Super-multiplex vibrational imaging. Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
Mechanical signaling coordinates the embryonic heartbeat. Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ. Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
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