Anti-Rabbit IgG F(ab) ATTO647N (H+L)

Product name

Anti-Rabbit IgG F(ab) ATTO647N 

Target species

Rabbit

Description

ATTO-labeled F(ab) fragments belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Rabbit IgG F(ab)-ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.

Conjugates of F(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access;
- prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in  Fab fragments;

Specification

Goat anti-rabbit IgG F(ab) ATTO647N (H+L), Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence. 

For further information please see DataSheet. 

Optical properties

λex 645nm, λem 669nm

Form

Lyophilized

Nominal concentration

2mg/ml

Content

 
Buffer

0.25mg Anti-Rabbit IgG (Fab) ATTO647N
1.0ml Glycerol buffer
Product documentation

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

Column affinity purified, IEX chromatography after papain cleavage.

Storage instructions

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping conditions 

Shipped at ambient temperature.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. 

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