ATTO-labeled F(ab) fragments belong to the new generation of fluorescent antibodies, characterized by their exceptional fluorescence intensity and superior photostability. Anti-Rabbit IgG F(ab)-ATTO647N is specifically suited for high resolution micoscopy, due to the high photostabilty and strong fluorescence quantum yield.
Conjugates of F(ab) fragments are preferable to whole antibody conjugates:
- for cellular structures which are sterically difficult to access; - prevention of interactions with Fc receptor-bearing membranes due to the absence of the Fc region in Fab fragments;
Specification
Goat anti-rabbit IgG F(ab) ATTO647N (H+L), Immunofluorescence: 1:300-1:500, Degree of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence.
Mechanical signaling coordinates the embryonic heartbeat. Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ. Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.
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