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Ubiquitin-ATTO488 - 200µg

Cat. #:
8421-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
200 µg
 

Ubiquitin-ATTO488
human, recombinant 

Cat. #: 8421-01t

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Applications

Fluorescence microscopy, FRET, Fluorescence spectroscopy, Biochemical assays.

 

Description

 

 

Protein

Ubiquitin-ATTO488

Origin

human, recombinant

Molecular mass

9.2kDa

Protein description

Ubiquitin is a ubiquitos regulatory protein (8.5kDa) that can be conjugated to substrate proteins by subsequent actions of enzymes, such as:: E1 - class of Ubiquitin  activating enzymes, E2 - class of Ubiquitin  conjugating enzymes and E3- class of Ubiquitin ligases, All assays have to be optimized for the individual set up and it is recommended to start with initial concentration of Ubiquitin-ATTO488 of 0.01-0.5mM. 


ATTO488-labeled ubiquitin is used as a general research tool or in high throughput assays (HTS) to monitor protein ubiquitination. The labelling method of the ubiquitin protein keeps all lysines functional for further and guarantees the full function of the protein.

Ubiquitin-ATTO488 (λex 501nm,λem 523nm). 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of ~1. 

 

Properties

 

 

Form

Lyophilized, ready-to-use.

Quantity  per unit

200 µg

Buffer

20mM TES pH 7.4, 50mM KCl and 0.3% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC 

Protein concentration

Determined by the Biuret method.

Storage instructions

Ubiquitin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ubiquitin is kept on ice and should be used within 2 weeks. Avoid refreezing.  

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 

 

 

Further Information


Product DataSheet

Material and Safety Data Sheet

 Table of Dyes and Light Sources 

Protein Sequence on NCBI





References
 

The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction.
Glickman MH, Ciechanover A.; Physiol Rev. 2002 Apr;82(2):373-428. Review.


Structure and functions of the 20S and 26S proteasomes.
Coux O, Tanaka K, Goldberg AL.; Annu Rev Biochem. 1996;65:801-47. Review.
 

Characterization of the heat-stable polypeptide of the ATP-dependent proteolytic system from reticulocytes.
Ciechanover A, Elias S, Heller H, Ferber S, Hershko A.; J Biol Chem. 1980 Aug 25;255(16):7525-8.