Protein

Ubiquitin-ATTO565

Origin

human, recombinant

Molecular mass

9.2kDa

Protein description

Ubiquitin is a ubiquitos regulatory protein (8.5kDa) that can be conjugated to substrate proteins by subsequent actions of enzymes, such as:: E1 - class of Ubiquitin  activating enzymes, E2 - class of Ubiquitin  conjugating enzymes and E3- class of Ubiquitin ligases, All assays have to be optimized for the individual set up and it is recommended to start with initial concentration of Ubiquitin-ATTO565 of 0.01-0.5mM.

ATTO565-labeled ubiquitin is used as a general research tool or in high throughput assays (HTS) to monitor protein ubiquitination. The labelling method of the ubiquitin protein keeps all lysines functional for further and guarantees the full function of the protein.

Ubiquitin-ATTO565 (λex 563nm,λem 592nm). 

Conjugation ratio

This product is supplied with a degree of labelling (DOL) of ~ 1.

Form

Lyophilized, ready-to-use.

Quantity per unit

200 µg

Buffer

20mM TES pH 7.4, 50mM KCl and 0.3% disaccharides, when reconstituted with 200 µl ultrapure water to obtain a 1mg/ml solution.

Purity

>99% by scanning densitometry.

Purification notes

Purified by GPC 

Protein concentration

Determined by the Biuret method.

Storage instructions

Ubiquitin is stored at –70°C upon arrival and will be stable in performance for at least 6 months from the date of purchase. Solubilized ubiquitin is kept on ice and should be used within 2 weeks. Avoid refreezing.  

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

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