Smooth muscle actin
(from porcine smooth muscle)
Cat. #: 8106-01
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Description
|
Protein |
Actin (smooth muscle actin) |
|
Origin |
porcine smooth muscle. |
|
Molecular mass |
42kDa |
|
Protein description |
Smooth muscle actin from gizzard contains a mixture of ~80% γ-, ~20% α- smooth muscle actin and a certain quantity of β-non-muscle actin. Smooth muscle actin is a single chain polypeptide with a molecular weight of 42kD consisting of 375 amino acids. The typical polymerization and depolymerization properties were compared with skeletal muscle actin in fluorimetrical analyses (see product images). By mixing both actins with 10% pyrene actin (rabbit skeletal muscle actin) different kinetics are observed. |
|
Purity |
>98% (determined by scanning densitometry). |
Properties
|
Form |
Lyophilized, ready-to-use. |
|
Quantity per unit |
2x250 µg |
|
Buffer |
2mM Tris-Cl pH 8.2, 1mM ATP, 0.5mM DTT, 0.1mM CaCl2 and 0.4% disaccharides, when reconstituted with H2O. |
|
Purity |
>98% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
|
Purification notes |
The native protein was purified by GPC from chicken gizzard. |
|
Protein concentration |
Determined by Biuret method. |
|
Storage instructions |
The protein is stored at –70°C upon arrival will be stable in performance for at least 6 months from the date of purchase. Avoid repeated freeze / thaw cycles. |
|
Shipping conditions |
At ambient temperature. Upon delivery store at -70°C. |
|
Remarks |
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications. |
|
CAS no. |
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI
References
Wang C, Becker K, Pfütze S, Kuhnert E, Stadler M, Cox RJ, Skellam E.Org Lett. 2019 Nov 1;21(21):8756-8760. doi: 10.1021/acs.orglett.9b03372. Epub 2019 Oct 23.PMID: 31644300
Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I.
Kern S, Feng HZ, Wei H, Cala S, Jin JP.FEBS Open Bio. 2013 Nov 23;4:11-7. doi: 10.1016/j.fob.2013.11.002. eCollection 2013.PMID: 24319652
Wang C, Becker K, Pfütze S, Kuhnert E, Stadler M, Cox RJ, Skellam E.Org Lett. 2019 Nov 1;21(21):8756-8760. doi: 10.1021/acs.orglett.9b03372. Epub 2019 Oct 23.PMID: 31644300
Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I.
Kern S, Feng HZ, Wei H, Cala S, Jin JP.FEBS Open Bio. 2013 Nov 23;4:11-7. doi: 10.1016/j.fob.2013.11.002. eCollection 2013.PMID: 24319652
