LDS-SampleBuffer (5x), Reducing - Ultrapure
High efficient sample buffer!
Cat. #:6159-01
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Description
LDS-Sample Buffer is a convenient sample buffer for use in SDS-PAGE. The additive lithiumdodecylsulfat (LDS) shows a significantly higher salt tolerance compared to sodiumdodecylsulafte (SDS). LDS-SampleBuffer is favored in preparing protein, cell or tissue samples at physiological salt concentration. In contrast to samples made up with SDS sample buffers frequently showing a white precipitate at room temperature, LDS samples are soluble at room temperature and also during cooled electrophoresis.
The buffer contains bromophenolblue dye as marker to vizualize the dye front during electrhoresis. The LDS Sample Buffer is used in denaturing gels and is compatible with coomassie blue (R-250 & G-250) and silver staining and Western blotting applications.
5x LDS-SampleBuffer (Tris-Cl pH 6.8) with 500mM DTT for use in denaturing SDS-PAGE. Includes DTT as a powder to be added freshly before use.
Unit size
10ml