Cofilin (non-muscle cofilin) - 2x50 µg
Cofilin
(Actin Binding Protein)
Description
| Protein | Cofilin (synonym: n-cofilin, cofilin-1) |
| Origin | Non-muscle isoform, murine recombinant (untagged) |
| Molecular mass | 18.5 kDa |
| Protein description | Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics and plays a role in the regulation of cell morphology and cytoskeletal organization. |
| Actin interaction | Non-muscle cofilin binds to G- and F-actin in a 1:1 molar ratio. |
Properties
| Form | Lyophilized, ready-to-use. |
| Quantity per unit | 2 × 50 µg |
| Buffer | 150 mM NaCl, 20 mM Hepes pH 7.4, 1 mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution. |
| Purity | >96% by scanning densitometry from Coomassie G-250 stained SDS gels. |
| Purification notes | Expressed as a GST-tag fusion protein in E. coli. After purification by GST-agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography. |
| Protein concentration | Determined by OD280 (0.1% = 0.778). |
| Storage instructions | Store at -70°C upon arrival; stable in performance for at least 6 months from date of purchase. Keep dissolved protein on ice during use; for longer storage store at -20°C in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze/thaw cycles. |
| Shipping conditions | At ambient temperature. Upon delivery store at -70°C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Material and Safety Data Sheet
Protein Sequence on NCBI (human non-muscle)
Protein Structures on RCSB (human non-muscle)
References
Initiation of DNA replication requires actin dynamics and formin activity.
Parisis N, Krasinska L, Harker B, Urbach S, Rossignol M, Camasses A, Dewar J, Morin N, Fisher D. EMBO J. 2017 Nov 2;36(21):3212-3231. doi: 10.15252/embj.201796585.
Solution structure of human cofilin: actin binding, pH sensitivity, and relationship to actin-depolymerizing factor.
Pope BJ, Zierler-Gould KM, Kühne R, Weeds AG, Ball LJ. J Biol Chem. 2004 Feb 6;279(6):4840-8.
