Goat Anti-Mouse IgM (H+L) ATTO565
Antibody produced in goat, affinity purified
Cat. #: 2207-1MG
Applications
Fluorescent secondary antibody for detection of mouse IgM in immunofluorescence microscopy and related fluorescence-based assays.
Description
| Product name | Goat Anti-Mouse IgM (H+L) ATTO565 | GXM IgM ATTO565 |
| Target species | Mouse |
| Description |
Goat Anti-Mouse IgM (H+L) ATTO565 is an antigen-specific fluorescent secondary antibody for detection of mouse IgM. Affinity purification removes essentially all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. The antibody is conjugated to ATTO565 and further purified by gel filtration to remove unbound dye and low-molecular-weight reaction components. ATTO565 has an absorption maximum at 563 nm and a fluorescence maximum at 592 nm. The antibody is supplied as a lyophilized preparation and can be reconstituted to a 2 mg/ml stock solution. Protect the fluorescent conjugate from light. |
| Specification | Immunofluorescence: 1:300–1:1000, Degree of Labeling (DOL): 2–5, Unbound dye ≤5% of total fluorescence. |
| Optical properties | λabs: 563 nm, λfl: 592 nm |
Properties
| Form | Lyophilized |
| Nominal concentration | 2 mg/ml after reconstitution with 0.5 ml glycerol buffer. |
| Content | 1.0 mg Goat Anti-Mouse IgM (H+L) ATTO565 20 µg monoclonal anti-actin reference antibody (1 mg/ml) 1.0 ml glycerol buffer supplied; add 0.5 ml to reconstitute the lyophilized secondary antibody. Final buffer: 50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, pH 7.4 |
| Clonality | Polyclonal |
| Target immunoglobulin | Mouse IgM (H+L) |
| Purification notes | Antigen-specific affinity purification removes essentially all goat serum proteins, including immunoglobulins not specifically binding to mouse IgM. Following conjugation to ATTO565, the antibody is further purified by gel filtration. |
| Storage instructions | After reconstitution, store at 2–8 °C for up to three months. For extended storage, aliquot and store at −20 °C; avoid repeated freeze/thaw cycles. Protect the fluorescent conjugate from light. |
| Shipping conditions | Shipped at ambient temperature. |
| Remarks | For research use only. Not for diagnostic, therapeutic, clinical or in vivo use. |
Buffers and antibodies from Hypermol® are made of the ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
References
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