Shopping Cart
no products
1 Products in this category

Actin-Toolkit F-Actin Binding (alpha skeletal muscle actin)

Cat. #:
8010-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Units:
1x kit

 


Actin-Toolkit F-Actin Binding
α-actin from rabbit skeletal muscle

Cat. #: 8010-01 

..............................................................................................................................................................................................................................................................................................................................................................................................................

 


Applications

-   Proof of the biological activity of a ligand by actin binding;

-   Cosedimentation analysis of ligands binding to F-actin;

-   Testing of actin filament bundling activity or network formation by ligands;

-   Analysis of pharmacological compounds, proteins or lipids altering ligand-actin-interaction;


 

Description

 

 

Protein

Actin (α-Actin)

Origin

Skeletal muscle alpha actin, rabbit

Molecular mass

42kDa

Toolkit description

Binding of ligands to filamentous actin is used as a functional proof to state the biological activity of a ligand and characterizes the ligand to be an actin binding protein. Specific actin binding indicates that the ligand is correctly folded and structurally intact. The Actin-Toolkit F-Actin Binding is a well-established and commonly used method to characterize ligand-actin interaction: at high speed centrifugation ligands binding to individual actin filaments are co-sedimented.

At low speed spinning a selective co-sedimentation of actin filament bundles or networks is achieved. Low speed assays instantly characterize ligands to induce actin suprastructures. 

The Actin-Toolkit F-Actin Binding was developed to characterize the actin-binding properties of ligands (native or recombinant proteins, recombinant fragments etc.) and to support the detection even of cryptic actin-binding sites. Further suitable applications are in vitro compound analysis of the ligand-actin interactions by investigating effects of pharmacological compounds on ligand-actin complexes, as well as the introduction of further ligands (proteins, lipids etc.) to ligand-actin complexes. 

The kit contains 4x1 mg 
skeletal muscle actin Cat. # 8010-03), buffers to keep actin functional and to prevent denaturing. A handbook introduces and guides through the experimental work with actin in F-actin Binding Assays.

Purity

>99% by scanning densitometry.




Properties

 

 

Form

Lyophilized, ready-to-use.

Kit content

4x1 mg Actin (lyophilized powder);
4x50 ml MonoMix (lyophilized G-actin ATP-buffer);
1x1 ml PolyMix, 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x1 ml PolyMix 6, 10x stock (lyophilized F-actin ATP-buffer, pH 6.0);
1x1 ml PolyMix 8, 10x stock (lyophilized F-actin ATP-buffer, pH 8.0);
1x0.5 ml MgCl2 (solution)
1xToolkit Handbook F-Actin Binding;

Buffer

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3% disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml solution.

Related products

For dilution of G-actin or exchange of ligand buffer into actin compatible buffer, MonoMix (Cat. #: 5100-0*) may be used. In the absence of nucleators, the polymerization of G-actin is initiated by PolyMix (Cat. #: 5000-0*). 

Purification notes

Purified from rabbit skeletal muscle, GPC.

Protein concentration

Determined by the Biuret method.

Storage instructions

We guarantee stable performance of the kit components for 6 months when stored at -70°C upon arrival. Solubilized G-actin is kept on ice and should be used within 1 week. Avoid refreezing.     

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.




 

Further Information
 

 Material and Safety Data Sheet

 

Protein Sequence on NCBI
 



 
Related products:

Gelsolin (cytoplasmic) - 2x50 µg

Gelsolin (cytoplasmic) - 2x50 µg

217,00 EUR
Profilin (human recombinant) - 2x50µg

Profilin (human recombinant) - 2x50µg

162,00 EUR
CapZ (non-muscle, human recombinant) - 2x50 µg

CapZ (non-muscle, human recombinant) - 2x50 µg

212,00 EUR
Customers who bought this product bought also the following products:

Actin-Toolkit G-Actin Binding (alpha skeletal muscle actin)

Actin-Toolkit G-Actin Binding (alpha skeletal...

382,00 EUR
Non-Muscle Actin - 1x200 µg

Non-Muscle Actin - 1x200 µg

from 176,00 EUR
Actin-Toolkit TIRFM (ATTO488-Actin)

Actin-Toolkit TIRFM (ATTO488-Actin)

647,00 EUR