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CytoStaining Kit 550-Phalloidin

Cat. #:
C8813-01
Shipping:
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CytoStaining Kit 550-Phalloidin
Suited for cultured cells & cell extracts


Cat. #: C8813-01   

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Applications


Rapid staining of actin filaments with highly photostable 550-Phalloidin. Suited for cultured cells & cell extracts.
Replacent for:
Alexa Fluor® 555, Cy™3,Tetramethylrhodamine.

 

Description

   

Product

550-Phalloidin (Atto550 phalloidin)

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid staining of single actin filaments with highly photostable 550-Phalloidin. Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita phalloides “death cap” mushroom. The peptide is commonly used as a selective stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of about one phallotoxin per actin subunit in both muscle and non-muscle cells, and have similar affinity for both large and small filaments. Phalloidins do not bind to monomeric actin and have a stabilizing effect on F-actin. 

550-Phalloidin has a strong pink fluorescence of high brightness and superior photostability.

Actin interaction

Selective staining of F-actin in fixed and permeabilized cells and cell free F-actin.



Description

   

Form

Dry

Quantity

1x 100 Units (sufficient for 100 slides)

Buffer

Reconstitute in 50µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with 500µl PBS and apply to stain  the sample.

Sub cellular localization

Actin, Cytoskeleton

Excitation/Emission

ex 554 nm, em 574 nm

Kit Content

1x 100 Units 550-Phalloidin
1x PBS tablet to make 100ml PBS 
1.0 ml FluoProtect 

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions 

At ambient temperature. Upon delivery store at -5 to -70°C.

Remarks

 

CAS no.

 N/A

Further Information


Table of Dyes and Light Sources