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CS-LB (Cytoskeleton LysisBuffer)

Cat. #:
5402-01
Shipping:
FedEx Express (Global) FedEx Express (Global) (shipping info)
Quantity:
1.0ml
Units:
1x
5x ( + 36,00 EUR )


CS-LB 
Cytoskeleton LysisBuffer

Cat. #: 5402-01

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Description

   

Product name

CS-LB (Cytoskeleton LysisBuffer)

Product description

CS-LB is a Lysis buffer to lyse cultured cells to specifically prepare cytoskeletal components. This includes soluble cytoskeleton fractions and also the insoluble, filamentous actin cytoskeleton. The buffer can be used solitary in assays of choice, but also in combination with many Actin-Toolkits (G-actin binding, ELISA etc.) to maximize the analytical applications of the kits. 

CS-LB is a complete, ready-to-use buffer with protease inhibitors included, specifically made for actin based assays and other cytoskeleton research areas.

Overview

CS-LB is used to:

  • prepare cell lysates for cytoskeleton research;
  • support the stability of cytoskeletal proteins;
  • prevent proteolytic degradation;



Properties

   

Form

Lyophilized, ready-to-use. Glycerol is not included and has to be added after reconstitution. 

Quantity

10x1 ml 

Buffer

Powder to make 10x1 ml of a CS-LB. Including 25 mM HEPES-KOH, pH 7.4, 100 mM KCl, 1 mM Mg2Cl, 1mM DTT, 10% glycerol, 2.5 mM Sodium orthovanadate, 2.5 mM Tetrasodium pyrophosphate, 1mM NaF, RNAse 10µg/ml, Protease-Inhibitors (AEBSF, leupeptin, E-64);

Purity

Ultrapure reagents in Milli-Q

Storage instructions

CS-LB is stored at -70°C upon arrival. After reconstititution, Aliquots are stored at -70°C, where the buffer will be stable in performance for at 6 months from the date of purchase. Avoid refreezing.    

Shipping conditions 

At ambient temperature. Upon delivery store at -70°C.

Remarks

For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.

CAS no.

 


Further Information

Product DataSheet

Material and Safety Data Sheet






 
Buffers from Hypermol are made of purest reagents in Milli-Q water, as described in our publications. 

Validated product quality with Satisfaction Guarantee! 





 
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