ATTO-labeled secondary antibodies belong to the new generation of fluorescent antibodies characterized by their exceptional fluorescence intensity and superior photostability. Anti-Mouse IgM ATTO532 is specifically suited for high resolution microscopy, due to the high photostability and strong fluorescence quantum yield.
For convenient reference, Fluorescent Antibody Kits contain gratis a primary epitope mapped actin antibody of high performance also on western blots.
Specification
Goat anti-mouse IgM ATTO532 (H+L), Immunofluorescence: 1:300-1:1000, Degree of labeling (DOL) : 2-9, Unconjugated dye ≤5% of total fluorescence.
For further information please see DataSheet.
Optical Properties
λex 532nm, λem 553nm
Properties
Form
Lyophilized
Nominal concentration
2 mg/ml
Content
Buffer
1 mg Anti-Mouse IgM (H+L) ATTO532 20 µg (1mg/ml) monoclonal anti-actin
1 ml Glycerol buffer Product documentation
Certificate of Analysis
50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 10 mM sodium azide, pH 7.4.
Clonality
Polyclonal
Isotype
IgM
Purification notes
Column affinity purified.
Storage instructions
Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.
Shipping conditions
Shipped at ambient temperature.
Remarks
For Use in Research only. Not for Use in Human or Veterinary Diagnostical or Therapeutical Applications.
CAS no.
Further Information
Product DataSheet
Material and Safety Data Sheet
Table of Dyes and Light Sources
Catalogue: ATTO Secondary Antibodies
References
Super-multiplex vibrational imaging. Wei L, Chen Z, Shi L, Long R, Anzalone AV, Zhang L, Hu F, Yuste R, Cornish VW, Min W.
Nature. 2017 Apr 27;544(7651):465-470. doi: 10.1038/nature22051. Epub 2017 Apr 19.
Mechanical signaling coordinates the embryonic heartbeat. Chiou KK, Rocks JW, Chen CY, Cho S, Merkus KE, Rajaratnam A, Robison P, Tewari M, Vogel K, Majkut SF, Prosser BL, Discher DE, Liu AJ. Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):8939-44. doi: 10.1073/pnas.1520428113. Epub 2016 Jul 25.