Actin-Toolkit Ligand Interaction
Actin-Toolkit Ligand Interaction
Actin-Toolkit Ligand Interaction

Actin-Toolkit Ligand Interaction

Cat. #:
8040-01
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517,00 EUR

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Description


Actin-Toolkit Ligand Interaction 
Rabbit Skeletal Muscle Alpha Actin

Cat. #: 8040-01

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Applications

  • Fluorescence analysis of native G-actin complexes;

  • Works with crude cell or tissue extracts;

  • A direct quick step assay without using antibodies to proof actin-ligand interactions; 



Description
 

The Actin-Toolkit Ligand Interaction facilitates fluorescent IAEDANS- conjugated actin to visualize interactions with actin ligands by native page. An advantage of this method is that also crude extracts or semi-purified proteins can be used. The assay leaves the interacting proteins in the native state and proofs the existence of a ligand-G-actin complex (see also
 
Actin-Toolkit G-Actin Binding). As this assay does not require the use of antibodies, results are obtained within a few steps and can observed using a 360nm UV-table.

The Actin-Toolkit Ligand Interaction contains all necessary components for error-free handling of the assay: 5x100µg IAEDANS-actin from rabbit skeletal muscle, buffers to keep the actin functional. A handbook guides through the experimental setup and contains our protocol for the preparation of native PA gels. The sample buffer (TrackingDye) for native PAGE is included. We guarantee stable performance of all kit components when stored at -70°C upon arrival for 6 months.


Kit Content (25-50 Assays)
 

5x100µg IAEDANS-Actin (lyophilized powder);
5x50ml MonoMix (lyophilized G-actin ATP-buffer);
1x1.0ml PolyMix 10x stock (lyophilized F-actin ATP-buffer, pH 7.4);
1x1.0ml TrackingDye;
1x Toolkit Handbook Ligand Interaction;
10x Black Tubes & Tags;  



Further Information
 

 Material and Safety Data Sheet  



 

This product is similar to:

CS-HSB (Cytoskeleton High Salt Buffer)
CS-HSB (Cytoskeleton High Salt Buffer)
CS-HSB is used to:
  • treatment of cell lysate derived preparations under highly stringent conditions;
from 104,00 EUR