ATTO550-Actin Skeletal muscle alpha-Actin (rabbit) 5x100µg
ATTO550-Actin
Fluorescent alpha-skeletal actin from rabbit skeletal muscle; lysine-labelled with ATTO550.
Applications
| Applications | Fluorescent, polymerization-competent alpha-skeletal actin for epifluorescence, high- and super-resolution microscopy, FRET, fluorescence spectroscopy, filament tracking and biochemical actin assays. |
Description
| Product name | ATTO550-Actin |
| Protein | Alpha-skeletal actin, rabbit. |
| Molecular mass | 42 kDa. |
| Product description | ATTO550-Actin is chemically modified rabbit skeletal G-actin carrying covalently attached ATTO550 fluorescent groups on lysine residues. The protein is prepared from alpha-skeletal actin isolated from rabbit skeletal muscle. Skeletal muscle actin is a single-chain polypeptide of approximately 42 kDa consisting of 375 amino acids. ATTO550-Actin is designed for fluorescence-based actin assays, epifluorescence microscopy, high-resolution microscopy and super-resolution microscopy, providing a defined fluorescent signal while retaining the polymerization properties required for actin-based biochemical workflows. |
| Fluorophore | ATTO550. |
| Spectral properties | Excitation maximum: 550 nm; emission maximum: 576 nm. |
| Labelling chemistry | ATTO550 is covalently attached to lysine residues of skeletal G-actin. |
| Degree of labelling | This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8. |
| Overview | Fluorescent rabbit skeletal G-actin; ATTO550-labelled on lysine residues; polymerization-competent; suitable for epifluorescence, high- and super-resolution microscopy, FRET, fluorescence spectroscopy and biochemical actin assays; supplied lyophilized in actin-compatible buffer. |
Properties
| Form | Lyophilized, ready-to-use after reconstitution. |
| Quantity per unit | 5 × 100 µg. |
| Reconstitution | Reconstitute each 100 µg tube with 100 µl ultrapure water to obtain a 1 mg/ml ATTO550-Actin solution. |
| Buffer | 2 mM Tris-HCl pH 8.2, 0.4 mM ATP, 0.5 mM DTT, 0.1 mM CaCl2, 1 mM NaN3 and 0.3% disaccharides after reconstitution with ultrapure water to obtain a 1 mg/ml solution. |
| Purity | >99% by scanning densitometry. |
| Purification notes | Purified by GPC from rabbit skeletal muscle. |
| Protein concentration | Determined by UV absorbance at 290 nm (A290 = 0.63 for 1 mg/ml actin) with fluorophore-specific correction factor applied. |
| Storage instructions | ATTO-Actins stored at −70 °C upon arrival will be stable in performance for at least 6 months from the date of purchase. Solubilized ATTO-Actin should be kept on ice and used within 1 week. Avoid refreezing. |
| Shipping conditions | At ambient temperature. Upon delivery store at −70 °C. |
| Remarks | For research use only. Not for use in human or veterinary diagnostic or therapeutic applications. |
| CAS no. | |
Proteins and buffers from Hypermol® are prepared with ultrapure reagents in Milli-Q™ water, as described in our publications.
Further Information
Product DataSheet
Download product datasheet for ATTO550-Actin.
Material and Safety Data Sheet
MSDS / SDS document for ATTO-labelled skeletal muscle actin products.
Protein Sequence on NCBI
Reference sequence information for rabbit skeletal alpha-actin.
Hide ATTO dyes & spectral properties
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ATTO dyes – spectral overview (λex / λem)
Hover/tap a dye to see details incl. possible excitation source.
References
Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation.
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R.
Nat Cell Biol. 2016 Sep;18(9):941-953. doi: 10.1038/ncb3402.
